Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta
Abstract
:1. Introduction
2. Results
2.1. PLET1 Gene Was Uniquely Expressed by Trophoblast Cells throughout Pig Placenta Development
2.2. The Location Pattern of PLET1 in Pig Trophoblast Cells Changes as the Pregnancy Proceeds
2.3. Two Different PLET1 Transcripts Were Identified to Be Expressed in Porcine Placenta by 3′RACE and Showed Different Expression Levels as the Placenta Developed
2.4. The Long and Short PLET1 3′ UTR Differentially Mediate the Luciferase Activity In Vitro
2.5. Investigation of the Interaction between Pig PLET1 and miR-365-3p In Vitro
2.6. miR-365-3p Was Differentially Expressed during Days 15, 26, 50, and 95 of Gestation in Pigs
2.7. PLET1 Is an N-Glycosylation Modified Protein
3. Discussion
4. Materials and Methods
4.1. Animals and Tissue Collections
4.2. Detection of the PLET1 mRNA Expression in Porcine Conceptus and Placenta by In Situ Hybridization
4.3. Investigation of PLET1 Protein Expression in Porcine Conceptus and Placenta by Immunohistochemistry
4.4. Investigation of the PLET1 Protein Expression in Porcine Conceptus and Placenta by Immunoflurescence
4.5. Cloning of the 5′and 3′ End of PLET1 Gene
4.6. Quantitative RT-PCR Analyses
4.7. Construction of Porcine PLET1 3′UTRs Luciferase Reporter Vector and Luciferase Reporter Assay
4.8. Validation of the Interaction between miR-365-3p and PLET1
4.9. Protein Enzymatic Deglycosylation by PNGase F and endoH
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Teng, L.; Hong, L.; Liu, R.; Chen, R.; Li, X.; Yu, M. Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta. Int. J. Mol. Sci. 2016, 17, 2048. https://doi.org/10.3390/ijms17122048
Teng L, Hong L, Liu R, Chen R, Li X, Yu M. Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta. International Journal of Molecular Sciences. 2016; 17(12):2048. https://doi.org/10.3390/ijms17122048
Chicago/Turabian StyleTeng, Liu, Linjun Hong, Ruize Liu, Ran Chen, Xinyun Li, and Mei Yu. 2016. "Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta" International Journal of Molecular Sciences 17, no. 12: 2048. https://doi.org/10.3390/ijms17122048