The Coexistence of Hypertension and Ovariectomy Additively Increases Cardiac Apoptosis
AbstractTo investigate whether the coexistence of hypertension and ovariectomy will increase cardiac Fas receptor and mitochondrial-dependent apoptotic pathways, histopathological analysis, the TUNEL assay and Western blotting were performed on the excised hearts from three groups of female spontaneously hypertensive rats (SHR), which were divided into a sham-operated group (SHR-Sham), bilaterally ovariectomized group (SHR-OVX) and normotensive Wistar Kyoto rats (WKY). Compared with the WKY group, the SHR-Sham group exhibited decreased protein levels of ERα, ERβ, p-Akt/Akt, Bcl-2, Bcl-xL and p-Bad and decreased further in the SHR-OVX group, as well as protein levels of t-Bid, Bak, Bad, Bax, cytochrome c, activated caspase-9 and activated caspase-3 (mitochondria-dependent apoptosis) increased in the SHR-Sham group and increased further in the SHR-OVX group. Compared with the WKY group, protein levels of Fas ligand, TNF-α, Fas death receptors, TNFR1, FADD and activated caspase-8 (Fas receptor-dependent apoptosis) increased in the SHR-Sham group, but did not increase in the SHR-OVX group, except Fas ligand and TNF-α. The coexistence of hypertension and ovariectomy attenuated the estrogen receptor survival pathway and appeared to additively increase the cardiac mitochondria-dependent, but not the Fas receptor-dependent apoptosis pathway, which might provide one possible mechanism for the development of cardiac abnormalities in hypertensive postmenopausal women. View Full-Text
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Lin, Y.-Y.; Cheng, Y.-J.; Hu, J.; Chu, L.-X.; Shyu, W.-C.; Kao, C.-L.; Lin, T.-B.; Kuo, C.-H.; Yang, A.-L.; Lee, S.-D. The Coexistence of Hypertension and Ovariectomy Additively Increases Cardiac Apoptosis. Int. J. Mol. Sci. 2016, 17, 2036.
Lin Y-Y, Cheng Y-J, Hu J, Chu L-X, Shyu W-C, Kao C-L, Lin T-B, Kuo C-H, Yang A-L, Lee S-D. The Coexistence of Hypertension and Ovariectomy Additively Increases Cardiac Apoptosis. International Journal of Molecular Sciences. 2016; 17(12):2036.Chicago/Turabian Style
Lin, Yi-Yuan; Cheng, Yu-Jung; Hu, Jun; Chu, Li-Xi; Shyu, Woei-Cherng; Kao, Chung-Lan; Lin, Tzer-Bin; Kuo, Chia-Hua; Yang, Ai-Lun; Lee, Shin-Da. 2016. "The Coexistence of Hypertension and Ovariectomy Additively Increases Cardiac Apoptosis." Int. J. Mol. Sci. 17, no. 12: 2036.
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