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Int. J. Mol. Sci. 2016, 17(12), 2035; doi:10.3390/ijms17122035

A Systematic Evaluation of Blood Serum and Plasma Pre-Analytics for Metabolomics Cohort Studies

1
Univ Lyon, CNRS, Université Claude Bernard Lyon 1, ENS de Lyon, Institut des Sciences Analytiques UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France
2
Centre Léon Bérard, Département de Recherche Translationnelle et de l’Innovation, 28 rue Laënnec, 69373 Lyon, CEDEX 08, France
3
Centre Léon Bérard, Département d’oncologie Médicale, 28 rue Laënnec, 69373 Lyon, CEDEX 08, France
4
Centre Léon Bérard, Département de Recherche Translationnelle et de l’Innovation, Génomique des Cancers, 28 rue Laënnec, 69373 Lyon, CEDEX 08, France
5
Department of Medical Oncology, Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, France
6
R&D UNICANCER, 101 rue de Tolbiac, 75654 Paris, CEDEX 13, France
*
Author to whom correspondence should be addressed.
Academic Editor: Elizabeth McKenzie
Received: 30 September 2016 / Revised: 14 November 2016 / Accepted: 29 November 2016 / Published: 5 December 2016
(This article belongs to the Special Issue Metabolomic Technologies in Medicine)
View Full-Text   |   Download PDF [935 KB, uploaded 5 December 2016]   |  

Abstract

The recent thriving development of biobanks and associated high-throughput phenotyping studies requires the elaboration of large-scale approaches for monitoring biological sample quality and compliance with standard protocols. We present a metabolomic investigation of human blood samples that delineates pitfalls and guidelines for the collection, storage and handling procedures for serum and plasma. A series of eight pre-processing technical parameters is systematically investigated along variable ranges commonly encountered across clinical studies. While metabolic fingerprints, as assessed by nuclear magnetic resonance, are not significantly affected by altered centrifugation parameters or delays between sample pre-processing (blood centrifugation) and storage, our metabolomic investigation highlights that both the delay and storage temperature between blood draw and centrifugation are the primary parameters impacting serum and plasma metabolic profiles. Storing the blood drawn at 4 °C is shown to be a reliable routine to confine variability associated with idle time prior to sample pre-processing. Based on their fine sensitivity to pre-analytical parameters and protocol variations, metabolic fingerprints could be exploited as valuable ways to determine compliance with standard procedures and quality assessment of blood samples within large multi-omic clinical and translational cohort studies. View Full-Text
Keywords: metabolomics; pre-analytics; nuclear magnetic resonance; serum; plasma; quality control metabolomics; pre-analytics; nuclear magnetic resonance; serum; plasma; quality control
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MDPI and ACS Style

Jobard, E.; Trédan, O.; Postoly, D.; André, F.; Martin, A.-L.; Elena-Herrmann, B.; Boyault, S. A Systematic Evaluation of Blood Serum and Plasma Pre-Analytics for Metabolomics Cohort Studies. Int. J. Mol. Sci. 2016, 17, 2035.

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