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Int. J. Mol. Sci. 2016, 17(12), 2033; doi:10.3390/ijms17122033

Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure

1
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China
2
Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, China
*
Author to whom correspondence should be addressed.
Academic Editor: Kun Yan Zhu
Received: 8 October 2016 / Revised: 23 November 2016 / Accepted: 29 November 2016 / Published: 3 December 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Pardosa pseudoannulata is one of the most common wandering spiders in agricultural fields and a potentially good bioindicator for heavy metal contamination. However, little is known about the mechanisms by which spiders respond to heavy metals at the molecular level. In the present study, high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and to identify differentially expressed genes (DEGs) after cadmium exposure. We obtained 60,489 assembled unigenes, 18,773 of which were annotated in the public databases. A total of 2939 and 2491 DEGs were detected between the libraries of two Cd-treated groups and the control. Functional enrichment analysis revealed that metabolism processes and digestive system function were predominately enriched in response to Cd stress. At the cellular and molecular levels, significantly enriched pathways in lysosomes and phagosomes as well as replication, recombination and repair demonstrated that oxidative damage resulted from Cd exposure. Based on the selected DEGs, certain critical genes involved in defence and detoxification were analysed. These results may elucidate the molecular mechanisms underlying spiders’ responses to heavy metal stress. View Full-Text
Keywords: P. pseudoannulata; cadmium; transcriptome; RT-qPCR P. pseudoannulata; cadmium; transcriptome; RT-qPCR
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, C.-C.; Wang, Y.; Li, G.-Y.; Yun, Y.-L.; Dai, Y.-J.; Chen, J.; Peng, Y. Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure. Int. J. Mol. Sci. 2016, 17, 2033.

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