Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure
AbstractPardosa pseudoannulata is one of the most common wandering spiders in agricultural fields and a potentially good bioindicator for heavy metal contamination. However, little is known about the mechanisms by which spiders respond to heavy metals at the molecular level. In the present study, high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and to identify differentially expressed genes (DEGs) after cadmium exposure. We obtained 60,489 assembled unigenes, 18,773 of which were annotated in the public databases. A total of 2939 and 2491 DEGs were detected between the libraries of two Cd-treated groups and the control. Functional enrichment analysis revealed that metabolism processes and digestive system function were predominately enriched in response to Cd stress. At the cellular and molecular levels, significantly enriched pathways in lysosomes and phagosomes as well as replication, recombination and repair demonstrated that oxidative damage resulted from Cd exposure. Based on the selected DEGs, certain critical genes involved in defence and detoxification were analysed. These results may elucidate the molecular mechanisms underlying spiders’ responses to heavy metal stress. View Full-Text
- Supplementary File 1:
ZIP-Document (ZIP, 1211 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Li, C.-C.; Wang, Y.; Li, G.-Y.; Yun, Y.-L.; Dai, Y.-J.; Chen, J.; Peng, Y. Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure. Int. J. Mol. Sci. 2016, 17, 2033.
Li C-C, Wang Y, Li G-Y, Yun Y-L, Dai Y-J, Chen J, Peng Y. Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure. International Journal of Molecular Sciences. 2016; 17(12):2033.Chicago/Turabian Style
Li, Chang-Chun; Wang, Yong; Li, Guo-Yuan; Yun, Yue-Li; Dai, Yu-Jun; Chen, Jian; Peng, Yu. 2016. "Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure." Int. J. Mol. Sci. 17, no. 12: 2033.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.