Int. J. Mol. Sci. 2016, 17(11), 1951; https://doi.org/10.3390/ijms17111951
Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment
Yali Liu 1
, Suying Han 2, Xiangming Ding 3, Xinmin Li 1,3, Lifeng Zhang 1, Wanfeng Li 1, Haiyan Xu 1, Zhexin Li 1 and Liwang Qi 1,*
, Suying Han 2, Xiangming Ding 3, Xinmin Li 1,3, Lifeng Zhang 1, Wanfeng Li 1, Haiyan Xu 1, Zhexin Li 1 and Liwang Qi 1,*
1
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2
Key Laboratory of Research Institute of Forest Ecology and Protection, Chinese Academy of Forestry, Beijing 100091, China
3
Department of Pathology and Laboratory Medicine, University of California at Los Angeles, 1000 Veteran Ave., Los Angeles, CA 90095, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Gian-Pietro Di Sansebastiano
Received: 27 September 2016 / Revised: 2 November 2016 / Accepted: 16 November 2016 / Published: 22 November 2016
(This article belongs to the Section Molecular Plant Sciences)
Abstract
Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as a bioactive molecule that can alleviate abiotic stress in plants. However, the biological effects of hydrogen in somatic embryos and the underlying molecular basis remain largely unknown. In this study, the morphological and physiological influence of exogenous H2 treatment during somatic embryogenesis was characterized in Larix leptolepis Gordon. The results showed that exposure to hydrogen increased the proportions of active pro-embryogenic cells and normal somatic embryos. We sequenced mRNA and microRNA (miRNA) libraries to identify global transcriptome changes at different time points during H2 treatment of larch pro-embryogenic masses (PEMs). A total of 45,393 mRNAs and 315 miRNAs were obtained. Among them, 4253 genes and 96 miRNAs were differentially expressed in the hydrogen-treated libraries compared with the control. Further, a large number of the differentially expressed mRNAs and miRNAs were related to reactive oxygen species (ROS) homeostasis and cell cycle regulation. We also identified 4399 potential target genes for 285 of the miRNAs. The differential expression data and the mRNA-miRNA interaction network described here provide new insights into the molecular mechanisms that determine the performance of PEMs exposed to H2 during somatic embryogenesis. View Full-TextKeywords:
hydrogen; somatic embryogenesis; pro-embryogenic mass; miRNA; reactive oxygen species (ROS) homeostasis; cell cycle
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Liu, Y.; Han, S.; Ding, X.; Li, X.; Zhang, L.; Li, W.; Xu, H.; Li, Z.; Qi, L. Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment. Int. J. Mol. Sci. 2016, 17, 1951.
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