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Int. J. Mol. Sci. 2016, 17(11), 1837; doi:10.3390/ijms17111837

Comparative Transcriptional Analysis of Loquat Fruit Identifies Major Signal Networks Involved in Fruit Development and Ripening Process

1
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai’an 223300, Jiangsu, China
2
Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai’an 223300, Jiangsu, China
*
Author to whom correspondence should be addressed.
Academic Editor: María Serrano
Received: 18 July 2016 / Revised: 24 October 2016 / Accepted: 26 October 2016 / Published: 4 November 2016
(This article belongs to the Special Issue Ripening Control and Induction of the Defence and Antioxidant Systems)
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Abstract

Loquat (Eriobotrya japonica Lindl.) is an important non-climacteric fruit and rich in essential nutrients such as minerals and carotenoids. During fruit development and ripening, thousands of the differentially expressed genes (DEGs) from various metabolic pathways cause a series of physiological and biochemical changes. To better understand the underlying mechanism of fruit development, the Solexa/Illumina RNA-seq high-throughput sequencing was used to evaluate the global changes of gene transcription levels. More than 51,610,234 high quality reads from ten runs of fruit development were sequenced and assembled into 48,838 unigenes. Among 3256 DEGs, 2304 unigenes could be annotated to the Gene Ontology database. These DEGs were distributed into 119 pathways described in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A large number of DEGs were involved in carbohydrate metabolism, hormone signaling, and cell-wall degradation. The real-time reverse transcription (qRT)-PCR analyses revealed that several genes related to cell expansion, auxin signaling and ethylene response were differentially expressed during fruit development. Other members of transcription factor families were also identified. There were 952 DEGs considered as novel genes with no annotation in any databases. These unigenes will serve as an invaluable genetic resource for loquat molecular breeding and postharvest storage. View Full-Text
Keywords: Eriobotrya japonica; transcriptome analysis; fruit development; ripening-regulated genes; expression profile Eriobotrya japonica; transcriptome analysis; fruit development; ripening-regulated genes; expression profile
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MDPI and ACS Style

Song, H.; Zhao, X.; Hu, W.; Wang, X.; Shen, T.; Yang, L. Comparative Transcriptional Analysis of Loquat Fruit Identifies Major Signal Networks Involved in Fruit Development and Ripening Process. Int. J. Mol. Sci. 2016, 17, 1837.

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