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Int. J. Mol. Sci. 2016, 17(10), 1719; doi:10.3390/ijms17101719

Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma

1
Faculty of Science, University of Ostrava, Ostrava 70103, Czech Republic
2
Department of Nutrition, Food Science and Gastronomy-XARTA-INSA, School of Pharmacy and Food Science, University of Barcelona, Barcelona 08028, Spain
3
CIBER CB06/03 Fisiopatología de la Obesidad y la Nutrición, CIBEROBN, Madrid 28049, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Battino
Received: 30 June 2016 / Revised: 26 September 2016 / Accepted: 9 October 2016 / Published: 14 October 2016
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [932 KB, uploaded 14 October 2016]   |  

Abstract

The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce. A new high pressure liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the quantification of selected carotenoids and fat-soluble vitamins in human plasma was developed, validated, and then applied in a pilot dietary intervention study with healthy volunteers. In 50 min, 16 analytes were separated with an excellent resolution and suitable MS signal intensity. The proposed HPLC–MS/MS method led to improvements in the limits of detection (LOD) and quantification (LOQ) for all analyzed compounds compared to the most often used HPLC–DAD methods, in some cases being more than 100-fold lower. LOD values were between 0.001 and 0.422 µg/mL and LOQ values ranged from 0.003 to 1.406 µg/mL, according to the analyte. The accuracy, precision, and stability met with the acceptance criteria of the AOAC (Association of Official Analytical Chemists) International. According to these results, the described HPLC-MS/MS method is adequately sensitive, repeatable and suitable for the large-scale analysis of compounds in biological fluids. View Full-Text
Keywords: tandem mass spectrometry; carotenoids; fat-soluble vitamins; human plasma; pilot human study; high antioxidant diet tandem mass spectrometry; carotenoids; fat-soluble vitamins; human plasma; pilot human study; high antioxidant diet
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hrvolová, B.; Martínez-Huélamo, M.; Colmán-Martínez, M.; Hurtado-Barroso, S.; Lamuela-Raventós, R.M.; Kalina, J. Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma. Int. J. Mol. Sci. 2016, 17, 1719.

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