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Int. J. Mol. Sci. 2016, 17(10), 1690; doi:10.3390/ijms17101690

Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino

1
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
2
Department of Horticulture, Agricultural College Yanbian University, Park Road 977, Yanji 133002, China
*
Author to whom correspondence should be addressed.
Academic Editor: Lam-Son Phan Tran
Received: 3 August 2016 / Revised: 26 September 2016 / Accepted: 29 September 2016 / Published: 9 October 2016
(This article belongs to the Section Molecular Botany)
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Abstract

Autopolyploidy is widespread in higher plants and plays an important role in the process of evolution. The present study successfully induced autotetraploidys from Chrysanthemum lavandulifolium by colchicine. The plant morphology, genomic, transcriptomic, and epigenetic changes between tetraploid and diploid plants were investigated. Ligulate flower, tubular flower and leaves of tetraploid plants were greater than those of the diploid plants. Compared with diploid plants, the genome changed as a consequence of polyploidization in tetraploid plants, namely, 1.1% lost fragments and 1.6% novel fragments occurred. In addition, DNA methylation increased after genome doubling in tetraploid plants. Among 485 common transcript-derived fragments (TDFs), which existed in tetraploid and diploid progenitors, 62 fragments were detected as differentially expressed TDFs, 6.8% of TDFs exhibited up-regulated gene expression in the tetraploid plants and 6.0% exhibited down-regulation. The present study provides a reference for further studying the autopolyploidization role in the evolution of C. lavandulifolium. In conclusion, the autopolyploid C. lavandulifolium showed a global change in morphology, genome and gene expression compared with corresponding diploid. View Full-Text
Keywords: Chrysanthemum lavandulifolium; morphology; SRAP (sequence-related amplified polymorphism); AFLP (amplified fragment length polymorphism); MSAP (methylation sensitive amplified polymorphism) Chrysanthemum lavandulifolium; morphology; SRAP (sequence-related amplified polymorphism); AFLP (amplified fragment length polymorphism); MSAP (methylation sensitive amplified polymorphism)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Gao, R.; Wang, H.; Dong, B.; Yang, X.; Chen, S.; Jiang, J.; Zhang, Z.; Liu, C.; Zhao, N.; Chen, F. Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino. Int. J. Mol. Sci. 2016, 17, 1690.

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