Next Article in Journal
Radiological Patterns of Brain Metastases in Breast Cancer Patients: A Subproject of the German Brain Metastases in Breast Cancer (BMBC) Registry
Next Article in Special Issue
Plasticizing Effects of Polyamines in Protein-Based Films
Previous Article in Journal
APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome
Previous Article in Special Issue
The Ultimaster Biodegradable-Polymer Sirolimus-Eluting Stent: An Updated Review of Clinical Evidence
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(10), 1608; doi:10.3390/ijms17101608

Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998

1
Fungal Biology Post-graduation Program, Federal University of Pernambuco, 50670-901 Recife-PE, Brazil
2
Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife-PE, Brazil
3
Biological Sciences Post-graduation Program, Federal University of Pernambuco, 50670-420 Recife-PE, Brazil
4
Northeast Network for Biotechnology Post-graduation Program, Federal Rural University of Pernambuco, 52171-900 Recife-PE, Brazil
5
National Post-Doctorate Program-CAPES, Catholic University of Pernambuco, 50050-900 Recife-PE, Brazil
6
Department of Antibiotics, Federal University of Pernambuco, 50670-901 Recife-PE, Brazil
*
Author to whom correspondence should be addressed.
Academic Editors: Carl J. Schaschke and Jean-Luc Audic
Received: 27 June 2016 / Revised: 2 September 2016 / Accepted: 6 September 2016 / Published: 23 September 2016
(This article belongs to the Special Issue Biodegradable Materials 2017)
View Full-Text   |   Download PDF [1630 KB, uploaded 8 October 2016]   |  

Abstract

Almost all oleaginous microorganisms are available for biodiesel production, and for the mechanism of oil accumulation, which is what makes a microbial approach economically competitive. This study investigated the potential that the yeast Candida lipolytica UCP0988, in an anamorphous state, has to produce simultaneously a bioemulsifier and to accumulate lipids using inexpensive and alternative substrates. Cultivation was carried out using waste soybean oil and corn steep liquor in accordance with 22 experimental designs with 1% inoculums (107 cells/mL). The bioemulsifier was produced in the cell-free metabolic liquid in the late exponential phase (96 h), at Assay 4 (corn steep liquor 5% and waste soybean oil 8%), with 6.704 UEA, IE24 of 96.66%, and showed an anionic profile. The emulsion formed consisted of compact small and stable droplets (size 0.2–5 µm), stable at all temperatures, at pH 2 and 4, and 2% salinity, and showed an ability to remove 93.74% of diesel oil from sand. The displacement oil (ODA) showed 45.34 cm2 of dispersion (central point of the factorial design). The biomass obtained from Assay 4 was able to accumulate lipids of 0.425 g/g biomass (corresponding to 42.5%), which consisted of Palmitic acid (28.4%), Stearic acid (7.7%), Oleic acid (42.8%), Linoleic acid (19.0%), and γ-Linolenic acid (2.1%). The results showed the ability of C. lipopytica to produce both bioemulsifier and biodiesel using the metabolic conversion of waste soybean oil and corn steep liquor, which are economic renewable sources. View Full-Text
Keywords: Candida lipolytica; agro-industrial waste; bioemulsifier; renewable sources; lipids; biofuel Candida lipolytica; agro-industrial waste; bioemulsifier; renewable sources; lipids; biofuel
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Souza, A.F.; Rodriguez, D.M.; Ribeaux, D.R.; Luna, M.A.C.; Lima e Silva, T.A.; Andrade, R.F.S.; Gusmão, N.B.; Campos-Takaki, G.M. Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998. Int. J. Mol. Sci. 2016, 17, 1608.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top