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Int. J. Mol. Sci. 2016, 17(1), 94; doi:10.3390/ijms17010094

Comparative Proteomic Analysis of Mature and Immature Oocytes of the Swamp Buffalo (Bubalus bubalis)

1
State Key Laboratory of Subtropical Agro-Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, Guangxi, China
2
Reproductive Medicine Center, Xiamen 361000, Fujian, China
*
Author to whom correspondence should be addressed.
Academic Editors: David Sheehan and Bing Yan
Received: 17 November 2015 / Revised: 16 December 2015 / Accepted: 8 January 2016 / Published: 14 January 2016
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

Maternal protein components change markedly during mammalian oogenesis. Many of these proteins have yet to be characterized and verified. In this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the Swamp Buffalo (Bubalus bubalis). Proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. Image analysis indicated that 17 proteins were differentially expressed between the two groups. Eight proteins were identified by mass spectrometry. In mature oocytes, three proteins were down-regulated: major vault protein (MVP), N-acetyllactosaminide β-1,6-N-acetylglucosaminyl-transferase (GCNT-2), and gem-associated protein (GEMIN)8, whereas five other proteins, heat shock protein (HSP)60, Ras-responsive element-binding protein 1 (RREB-1), heat shock cognate 71 kDa protein (HSC71), hemoglobin subunit α (HBA), and BMP-2-inducible protein kinase (BMP-2K), were up-regulated. The expression profiles of HSP60 and GEMIN8 were further verified by Western blotting. The changes in HSP60 protein expression demonstrate the increasing need for mitochondrial protein importation to facilitate macromolecular assembly during oocyte maturation. The down-regulation of GEMIN8 production implies that RNA splicing is impaired in mature oocytes. View Full-Text
Keywords: swamp buffalo; oocyte; comparative proteomics; heat shock protein 60 (HSP60); Gem-associated Protein 8 (GEMIN8); Western blotting swamp buffalo; oocyte; comparative proteomics; heat shock protein 60 (HSP60); Gem-associated Protein 8 (GEMIN8); Western blotting
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fu, Q.; Liu, Z.-F.; Huang, Y.-L.; Lu, Y.-Q.; Zhang, M. Comparative Proteomic Analysis of Mature and Immature Oocytes of the Swamp Buffalo (Bubalus bubalis). Int. J. Mol. Sci. 2016, 17, 94.

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