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Int. J. Mol. Sci. 2016, 17(1), 68; doi:10.3390/ijms17010068

Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting

Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea
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Academic Editors: Hitoshi Sashiwa, Qinghua Qin and Antonella Piozzi
Received: 25 September 2015 / Revised: 23 December 2015 / Accepted: 30 December 2015 / Published: 7 January 2016
(This article belongs to the Special Issue Chitins 2015)
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Abstract

The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto the surfaces of nanofibers using a peptide-coupling agent, and evaluated its biological effects on stem cells. The amount of immobilized SP on chitosan nanofibers was modulated over the range of 5.89 ± 3.27 to 75.29 ± 24.31 ng when reacted with 10 to 500 ng SP. In vitro migration assays showed that SP-incorporated nanofibers induced more rapid migration of human mesenchymal stem cells on nanofibers compared to pristine samples. Finally, the conjugated SP evoked a minimal foreign body reaction and recruited a larger number of CD29- and CD44-positive stem cells into nanofibers in a mouse subcutaneous pocket model. View Full-Text
Keywords: chitosan; substance-P; wound healing; electrospinning; human mesenchymal stem cell; tissue engineering chitosan; substance-P; wound healing; electrospinning; human mesenchymal stem cell; tissue engineering
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MDPI and ACS Style

Kim, M.S.; Park, S.J.; Cho, W.; Gu, B.K.; Kim, C.-H. Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting. Int. J. Mol. Sci. 2016, 17, 68.

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