Next Article in Journal
Technological Implications of Modifying the Extent of Cell Wall-Proanthocyanidin Interactions Using Enzymes
Previous Article in Journal
Metformin Restores Parkin-Mediated Mitophagy, Suppressed by Cytosolic p53
Previous Article in Special Issue
Altered Pre-mRNA Splicing Caused by a Novel Intronic Mutation c.1443+5G>A in the Dihydropyrimidinase (DPYS) Gene
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(1), 121; doi:10.3390/ijms17010121

Alternative Splicing in Adhesion- and Motility-Related Genes in Breast Cancer

1
Institute of Genetics and Biophysics “Adriano Buzzati-Traverso”, Consiglio Nazionale delle Ricerche, Via P. Castellino 111, 80131 Naples, Italy
2
Lab of Experimental Oncology and Pharmacogenomics, Istituto di Ricovero e Cura a Carattere Scientifico Fondazione “Salvatore Maugeri”, 27100 Pavia, Italy
3
Medical Oncology Unit, Azienda Ospedaliera Papa Giovanni XXIII, 24127 Bergamo, Italy
4
Department of Science and Technology, University Parthenope of Naples, 80131 Naples, Italy
5
Institute of Protein Biochemistry, Consiglio Nazionale delle Ricerche, Via P. Castellino 111, 80131 Naples, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Akila Mayeda and William Chi-shing Cho
Received: 27 July 2015 / Revised: 6 January 2016 / Accepted: 6 January 2016 / Published: 16 January 2016
(This article belongs to the Special Issue Pre-mRNA Splicing 2015)
View Full-Text   |   Download PDF [6977 KB, uploaded 16 January 2016]   |  

Abstract

Breast cancer is the most common tumor and the second leading cause of cancer death among woman, mainly caused by the metastatic spread. Tumor invasiveness is due to an altered expression of adhesion molecules. Among them, semaphorins are of peculiar interest. Cancer cells can manipulate alternative splicing patterns to modulate the expression of adhesion- and motility-related molecules, also at the isoform level. In this study, combining RNA-Sequencing on MCF-7 to targeted experimental validations—in human breast cell lines and breast tumor biopsies—we identified 12 new alternative splicing transcripts in genes encoding adhesion- and motility-related molecules, including semaphorins, their receptors and co-receptors. Among them, a new SEMA3F transcript is expressed in all breast cell lines and breast cancer biopsies, and is translated into a new semaphorin 3F isoform. In silico analysis predicted that most of the new putative proteins lack functional domains, potentially missing some functions and acquiring new ones. Our findings better describe the extent of alternative splicing in breast cancer and highlight the need to further investigate adhesion- and motility-related molecules to gain insights into breast cancer progression. View Full-Text
Keywords: alternative splicing; breast cancer; RNA-Sequencing; cell adhesion and motility alternative splicing; breast cancer; RNA-Sequencing; cell adhesion and motility
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Aversa, R.; Sorrentino, A.; Esposito, R.; Ambrosio, M.R.; Amato, A.; Zambelli, A.; Ciccodicola, A.; D’Apice, L.; Costa, V. Alternative Splicing in Adhesion- and Motility-Related Genes in Breast Cancer. Int. J. Mol. Sci. 2016, 17, 121.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top