Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs
Abstract
:1. Introduction
2. Results
2.1. Detection of the Differentially Expressed Genes
2.2. Identification of Genes Required for the Embryo Implantation and Placentation in Porcine Endometrium
2.3. Pregnancy Causes Distinct Transcriptome Changes between Early and Mid-Gestation in Meishan Endometrium
2.4. Pregnancy Causes Unique Alteration in the Transcriptome of Yorkshire Endometrium
2.5. Verification of the Gene Expression Patterns by Quantitative RT-PCR
3. Discussion
4. Experimental Section
4.1. Animals and Tissue Collection
4.2. Porcine Affymetrix GeneChip Hybridization and Data Analysis
4.3. Clustering Analysis
4.4. Gene Ontology (GO) and KEGG Pathway Analysis
4.5. Quantitative RT-PCR for DEGs Validation
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Huang, J.; Liu, R.; Su, L.; Xiao, Q.; Yu, M. Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs. Int. J. Mol. Sci. 2015, 16, 22692-22710. https://doi.org/10.3390/ijms160922692
Huang J, Liu R, Su L, Xiao Q, Yu M. Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs. International Journal of Molecular Sciences. 2015; 16(9):22692-22710. https://doi.org/10.3390/ijms160922692
Chicago/Turabian StyleHuang, Jiangnan, Ruize Liu, Lijie Su, Qian Xiao, and Mei Yu. 2015. "Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs" International Journal of Molecular Sciences 16, no. 9: 22692-22710. https://doi.org/10.3390/ijms160922692