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Int. J. Mol. Sci. 2015, 16(9), 21363-21377; doi:10.3390/ijms160921363

Ile-1781-Leu and Asp-2078-Gly Mutations in ACCase Gene, Endow Cross-resistance to APP, CHD, and PPZ in Phalaris minor from Mexico

1
Bayer CropScience, Col. Ampl. Granada
2
Department of Agricultural Chemistry and Edaphology, University of Cordoba, 14071 Cordoba, Spain
3
Deparment of Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, 49189-43464 Gorgan, Iran
4
Finca La Orden-Valdesequera Research Centre, 06187 Badajoz, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Lam-Son Phan Tran
Received: 21 July 2015 / Revised: 11 August 2015 / Accepted: 21 August 2015 / Published: 7 September 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Herbicides that inhibit acetyl coenzyme A carboxylase (ACCase) are commonly used in Mexico to control weedy grasses such as little seed canarygrass (Phalaris minor). These herbicides are classified into three major families (ariloxyphenoxypropionates (APP), cyclohexanodiones (CHD), and, recently, phenylpyrazolines (PPZ)). In this work, the resistance to ACCase (APP, CHD, and PPZ) inhibiting herbicides was studied in a biotype of Phalaris minor (P. minor) from Mexico, by carrying out bioassays at the whole-plant level and investigating the mechanism behind this resistance. Dose-response and ACCase in vitro activity assays showed cross-resistance to all ACCase herbicides used. There was no difference in the absorption, translocation, and metabolism of the 14C-diclofop-methyl between the R and S biotypes. The PCR generated CT domain fragments of ACCase from the R biotype and an S reference were sequenced and compared. The Ile-1781-Leu and Asp-2078-Gly point mutations were identified. These mutations could explain the loss of affinity for ACCase by the ACCase-inhibing herbicides. This is the first report showing that this substitution confers resistance to APP, CHD, and PPZ herbicides in P. minor from Mexico. The mutations have been described previously only in a few cases; however, this is the first study reporting on a pattern of cross-resistance with these mutations in P. minor. The findings could be useful for better management of resistant biotypes carrying similar mutations. View Full-Text
Keywords: canarygrass; APP; CHD; PPZ; NTSR; TSR; mutation resistance canarygrass; APP; CHD; PPZ; NTSR; TSR; mutation resistance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cruz-Hipolito, H.; Fernandez, P.; Alcantara, R.; Gherekhloo, J.; Osuna, M.D.; De Prado, R. Ile-1781-Leu and Asp-2078-Gly Mutations in ACCase Gene, Endow Cross-resistance to APP, CHD, and PPZ in Phalaris minor from Mexico. Int. J. Mol. Sci. 2015, 16, 21363-21377.

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