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Int. J. Mol. Sci. 2015, 16(9), 20657-20673; doi:10.3390/ijms160920657

A Novel Sucrose-Regulatory MADS-Box Transcription Factor GmNMHC5 Promotes Root Development and Nodulation in Soybean (Glycine max [L.] Merr.)

1
The National Key Facility for Crop Gene Resources and Genetic Improvement and MOA Key Lab of Soybean Biology (Beijing), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Haidian District, Beijing 100081, China
2
School of Life Science, Beijing Institute of Technology, 5 Zhongguancun South Street, Haidian District, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 8 May 2015 / Revised: 3 August 2015 / Accepted: 18 August 2015 / Published: 31 August 2015
(This article belongs to the Special Issue Plant Molecular Biology)
View Full-Text   |   Download PDF [1918 KB, uploaded 31 August 2015]   |  

Abstract

The MADS-box protein family includes many transcription factors that have a conserved DNA-binding MADS-box domain. The proteins in this family were originally recognized to play prominent roles in floral development. Recent findings, especially with regard to the regulatory roles of the AGL17 subfamily in root development, have greatly broadened their known functions. In this study, a gene from soybean (Glycine max [L.] Merr.), GmNMHC5, was cloned from the Zigongdongdou cultivar and identified as a member of the AGL17 subfamily. Real-time fluorescence quantitative PCR analysis showed that GmNMHC5 was expressed at much higher levels in roots and nodules than in other organs. The activation of expression was first examined in leaves and roots, followed by shoot apexes. GmNMHC5 expression levels rose sharply when the plants were treated under short-day conditions (SD) and started to pod, whereas low levels were maintained in non-podding plants under long-day conditions (LD). Furthermore, overexpression of GmNMHC5 in transgenic soybean significantly promoted lateral root development and nodule building. Moreover, GmNMHC5 is upregulated by exogenous sucrose. These results indicate that GmNMHC5 can sense the sucrose signal and plays significant roles in lateral root development and nodule building. View Full-Text
Keywords: Glycine max; GmNMHC5; lateral roots development; MADS-box protein; sucrose; nodule building Glycine max; GmNMHC5; lateral roots development; MADS-box protein; sucrose; nodule building
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, W.; Han, X.; Zhan, G.; Zhao, Z.; Feng, Y.; Wu, C. A Novel Sucrose-Regulatory MADS-Box Transcription Factor GmNMHC5 Promotes Root Development and Nodulation in Soybean (Glycine max [L.] Merr.). Int. J. Mol. Sci. 2015, 16, 20657-20673.

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