Next Article in Journal
Supracolloidal Assemblies as Sacrificial Templates for Porous Silk-Based Biomaterials
Next Article in Special Issue
Attenuation of Combined Nickel(II) Oxide and Manganese(II, III) Oxide Nanoparticles’ Adverse Effects with a Complex of Bioprotectors
Previous Article in Journal
Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms
Previous Article in Special Issue
New Hybrid Nanomaterial Based on Self-Assembly of Cyclodextrins and Cobalt Prussian Blue Analogue Nanocubes
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(9), 20492-20510; doi:10.3390/ijms160920492

Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration

1
School of Clinical Dentistry, University of Sheffield, Sheffield S10 2TA, UK
2
Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Turin 10129, Italy
3
School of Pharmacy, Royal College of Surgeons in Ireland, Dublin 2, Ireland
4
Division of Biology, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin 2, Ireland
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 21 July 2015 / Revised: 5 August 2015 / Accepted: 7 August 2015 / Published: 28 August 2015
(This article belongs to the Collection Bioactive Nanoparticles)
View Full-Text   |   Download PDF [1510 KB, uploaded 28 August 2015]   |  

Abstract

Biodegradable poly(lactide-co-glycolide) (PLGA) nanoparticles, containing human parathyroid hormone (PTH (1–34)), prepared by a modified double emulsion-solvent diffusion-evaporation method, were incorporated in porous freeze-dried chitosan-gelatin (CH-G) scaffolds. The PTH-loaded nanoparticles (NPTH) were characterised in terms of morphology, size, protein loading, release kinetics and in vitro assessment of biological activity of released PTH and cytocompatibility studies against clonal human osteoblast (hFOB) cells. Structural integrity of incorporated and released PTH from nanoparticles was found to be intact by using Tris-tricine SDS-PAGE. In vitro PTH release kinetics from PLGA nanoparticles were characterised by a burst release followed by a slow release phase for 3–4 weeks. The released PTH was biologically active as evidenced by the stimulated release of cyclic AMP from hFOB cells as well as increased mineralisation studies. in vitro and cell studies demonstrated that the PTH bioactivity was maintained during the fabrication of PLGA nanoparticles and upon release. Finally, a content of 33.3% w/w NPTHs was incorporated in CH-G scaffolds, showing an intermittent release during the first 10 days and, followed by a controlled release over 28 days of observation time. The increased expression of Alkaline Phosphatase levels on hFOB cells further confirmed the activity of intermittently released PTH from scaffolds. View Full-Text
Keywords: bone tissue; nanoparticles; parathyroid hormone; poly(lactide-co-glycolide); scaffolds bone tissue; nanoparticles; parathyroid hormone; poly(lactide-co-glycolide); scaffolds
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gentile, P.; Nandagiri, V.K.; Pabari, R.; Daly, J.; Tonda-Turo, C.; Ciardelli, G.; Ramtoola, Z. Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration. Int. J. Mol. Sci. 2015, 16, 20492-20510.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top