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Int. J. Mol. Sci. 2015, 16(8), 19713-19727; doi:10.3390/ijms160819713

Inhibitory Effects of Amorphigenin on the Mitochondrial Complex I of Culex pipiens pallens Coquillett (Diptera: Culicidae)

Department of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Vladimír Křen
Received: 28 May 2015 / Revised: 25 July 2015 / Accepted: 11 August 2015 / Published: 20 August 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Previous studies in our laboratory found that the extract from seeds of Amorpha fruticosa in the Leguminosae family had lethal effects against mosquito larvae, and an insecticidal compound amorphigenin was isolated. In this study, the inhibitory effects of amorphigenin against the mitochondrial complex I of Culex pipiens pallens (Diptera: Culicidae) were investigated and compared with that of rotenone. The results showed that amorphigenin and rotenone can decrease the mitochondrial complex I activity both in vivo and in vitro as the in vivo IC50 values (the inhibitor concentrations leading to 50% of the enzyme activity lost) were determined to be 2.4329 and 2.5232 μmol/L, respectively, while the in vitro IC50 values were 2.8592 and 3.1375 μmol/L, respectively. Both amorphigenin and rotenone were shown to be reversible and mixed-I type inhibitors of the mitochondrial complex I of Cx. pipiens pallens, indicating that amorphigenin and rotenone inhibited the enzyme activity not only by binding with the free enzyme but also with the enzyme-substrate complex, and the values of KI and KIS for amorphigenin were determined to be 20.58 and 87.55 μM, respectively, while the values for rotenone were 14.04 and 69.23 μM, respectively. View Full-Text
Keywords: Culex pipiens pallens; amorphigenin; mitochondrial complex I; inhibitory mechanism; kinetics Culex pipiens pallens; amorphigenin; mitochondrial complex I; inhibitory mechanism; kinetics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ji, M.; Liang, Y.; Gu, Z.; Li, X. Inhibitory Effects of Amorphigenin on the Mitochondrial Complex I of Culex pipiens pallens Coquillett (Diptera: Culicidae). Int. J. Mol. Sci. 2015, 16, 19713-19727.

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