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Int. J. Mol. Sci. 2015, 16(8), 17005-17017; doi:10.3390/ijms160817005

The Role of CzcRS Two-Component Systems in the Heavy Metal Resistance of Pseudomonas putida X4

1
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
2
Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Ji-Dong Gu
Received: 31 March 2015 / Revised: 16 June 2015 / Accepted: 30 June 2015 / Published: 27 July 2015
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Abstract

The role of different czcRS genes in metal resistance and the cross-link between czcRS and czcCBA in Pseudomonas putida X4 were studied to advance understanding of the mechanisms by which P. putida copes with metal stress. Similar to P. putida KT2440, two complete czcRS1 and czcRS2 two-component systems, as well as a czcR3 without the corresponding sensing component were amplified in P. putida X4. The histidine kinase genes czcS1 and czcS2 were inactivated and fused to lacZ by homologous recombination. The lacZ fusion assay revealed that Cd2+ and Zn2+ caused a decrease in the transcription of czcRS1, whereas Cd2+ treatment enhanced the transcription of czcRS2. The mutation of different czcRSs showed that all czcRSs are necessary to facilitate full metal resistance in P. putida X4. A putative gene just downstream of czcR3 is related to metal ion resistance, and its transcription was activated by Zn2+. Data from quantitative real-time polymerase chain reaction (qRT-PCR) strongly suggested that czcRSs regulate the expression of czcCBA, and a cross-link exists between different czcRSs. View Full-Text
Keywords: czcRS; two-component system; metal resistance; Pseudomonas putida czcRS; two-component system; metal resistance; Pseudomonas putida
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, P.; Chen, X.; Huang, Q.; Chen, W. The Role of CzcRS Two-Component Systems in the Heavy Metal Resistance of Pseudomonas putida X4. Int. J. Mol. Sci. 2015, 16, 17005-17017.

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