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Int. J. Mol. Sci. 2015, 16(7), 14884-14900; doi:10.3390/ijms160714884

Homogeneous Nature of Malaysian Marine Fish Epinephelus fuscoguttatus (Perciformes; Serranidae): Evidence Based on Molecular Markers, Morphology and Fourier Transform Infrared Analysis

1
Institute of Systems Biology, Universiti Kebangsaan Malaysia, UKM Bangi, 43600 Selangor, Malaysia
2
Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu Sabah, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 11 March 2015 / Revised: 4 June 2015 / Accepted: 17 June 2015 / Published: 2 July 2015
(This article belongs to the Special Issue Fish Molecular Biology)
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Abstract

Taxonomic confusion exists within the genus Epinephelus due to the lack of morphological specializations and the overwhelming number of species reported in several studies. The homogenous nature of the morphology has created confusion in the Malaysian Marine fish species Epinephelus fuscoguttatus and Epinephelus hexagonatus. In this study, the partial DNA sequence of the 16S gene and mitochondrial nucleotide sequences of two gene regions, Cytochrome Oxidase Subunit I and III were used to investigate the phylogenetic relationship between them. In the phylogenetic trees, E. fuscoguttatus was monophyletic with E. hexagonatus species and morphology examination shows that no significant differences were found in the morphometric features between these two taxa. This suggests that E. fuscoguttatus is not distinguishable from E. hexagonatus species, and that E. fuscoguttatus have been identified to be E. hexagonatus species is likely attributed to differences in environment and ability to camouflage themselves under certain conditions. Interestingly, this finding was also supported by Principal Component Analysis on Attenuated Total Reflectance–Fourier-transform Infrared (ATR-FTIR) data analysis. Molecular, morphological and meristic characteristics were combined with ATR-FTIR analysis used in this study offer new perspectives in fish species identification. To our knowledge, this is the first report of an extensive genetic population study of E. fuscoguttatus in Malaysia and this understanding will play an important role in informing genetic stock-specific strategies for the management and conservation of this highly valued fish. View Full-Text
Keywords: 16S; cytochrome oxidase; species identification; Epinephelus fuscoguttatus; Epinephelus hexagonatus 16S; cytochrome oxidase; species identification; Epinephelus fuscoguttatus; Epinephelus hexagonatus
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Nurdalila, A.A.; Bunawan, H.; Kumar, S.V.; Rodrigues, K.F.; Baharum, S.N. Homogeneous Nature of Malaysian Marine Fish Epinephelus fuscoguttatus (Perciformes; Serranidae): Evidence Based on Molecular Markers, Morphology and Fourier Transform Infrared Analysis. Int. J. Mol. Sci. 2015, 16, 14884-14900.

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