Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples
AbstractAn important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. In this work, a novel molecularly imprinted polymer composite membrane (MIM) was synthesized and employed for the selective detection in urine samples of 2-deoxyadenosine (2-dA), an important tumoral marker. By thermal polymerization, the 2-dA-MIM was cross-linked on the surface of a polyvinylidene-difluoride (PVDF) membrane. By characterization techniques, the linking of the imprinted polymer on the surface of the membrane was found. Batch-wise guest binding experiments confirmed the absorption capacity of the synthesized membrane towards the template molecule. Subsequently, a time-course of 2-dA retention on membrane was performed and the best minimum time (30 min) to bind the molecule was established. HPLC analysis was also performed to carry out a rapid detection of target molecule in urine sample with a recovery capacity of 85%. The experiments indicated that the MIM was highly selective and can be used for revealing the presence of 2-dA in urine samples. View Full-Text
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Scorrano, S.; Mergola, L.; Di Bello, M.P.; Lazzoi, M.R.; Vasapollo, G.; Del Sole, R. Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples. Int. J. Mol. Sci. 2015, 16, 13746-13759.
Scorrano S, Mergola L, Di Bello MP, Lazzoi MR, Vasapollo G, Del Sole R. Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples. International Journal of Molecular Sciences. 2015; 16(6):13746-13759.Chicago/Turabian Style
Scorrano, Sonia; Mergola, Lucia; Di Bello, Maria P.; Lazzoi, Maria R.; Vasapollo, Giuseppe; Del Sole, Roberta. 2015. "Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples." Int. J. Mol. Sci. 16, no. 6: 13746-13759.