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Int. J. Mol. Sci. 2015, 16(6), 12454-12466; doi:10.3390/ijms160612454

The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation

1
Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista-UNESP, CEP 18618-970 Botucatu, São Paulo, Brazil
2
Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista-UNESP, CEP 18618-970 Botucatu, São Paulo, Brazil
3
Centro Nacional de Pesquisa de Agroenergia (CNPAE), Empresa Brasileira de Pesquisa Agropecuária, CEP 70770-901, Brasília, Brazil
4
Faculdade de Ciências de Bauru, Universidade Estadual Paulista-UNESP, CEP 17033-360 Bauru, Brazil
5
Laboratório Especial de Toxinologia Aplicada. Butantan, Instituto Butantan, CEP 05503-900 São Paulo, Brazil
6
Campus Experimental do Litoral Paulista, Universidade Estadual Paulista-UNESP, CEP 11330-900 São Vicente, São Paulo, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Masatoshi Maki
Received: 27 April 2015 / Revised: 19 May 2015 / Accepted: 19 May 2015 / Published: 2 June 2015
(This article belongs to the Special Issue Metalloproteins)
View Full-Text   |   Download PDF [1306 KB, uploaded 2 June 2015]   |  

Abstract

Inflammation and haemorrhage are the main characteristics of tissue injury in botropic envenomation. Although some studies have shown that anti-venom prevents systemic reactions, it is not efficient in preventing tissue injury at the site of the bite. Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process. Effects of the extract and fractions from D. elliptica were evaluated using a carrageenan-induced paw oedema model in rats, and leukocyte rolling was visualized by intravital. The quantification of MMPs activities (MMP-2 and MMP-9) extracted from the dermis of mice treated with extract and fractions alone or incubated with venom was determined by zymographic analyses. Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge. Treatment with the tannins fraction also resulted in considerable inhibition of the rolling of leukocytes and this fraction was able to decrease the activation of MMP-9. These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity. View Full-Text
Keywords: Davilla elliptica St. Hil; anti-inflammatory effect; MMP-9; MMP-2; Bothrops jararaca venom Davilla elliptica St. Hil; anti-inflammatory effect; MMP-9; MMP-2; Bothrops jararaca venom
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nishijima, C.M.; Delella, F.K.; Rodrigues, C.M.; Rinaldo, D.; Lopes-Ferreira, M.V.A.; da Rocha, L.R.M.; Vilegas, W.; Felisbino, S.L.; Hiruma-Lima, C.A. The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation. Int. J. Mol. Sci. 2015, 16, 12454-12466.

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