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Int. J. Mol. Sci. 2015, 16(6), 12436-12453; doi:10.3390/ijms160612436

Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia

1
MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA
2
Department of Medicine, Division of Nephrology, Lehigh Valley Health Network, University of South Florida Morsani College of Medicine, Allentown, PA 18105, USA
3
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
4
Department of Obstetrics and Gynecology, University of Massachusetts Medical School/ UMass Memorial Health Care, Worcester, MA 01605, USA
5
Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA 01655, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Philip Newton Baker
Received: 1 April 2015 / Revised: 6 May 2015 / Accepted: 25 May 2015 / Published: 2 June 2015
(This article belongs to the Special Issue Prediction, Diagnostics and Prevention of Pregnancy Complications)
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Abstract

Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized. View Full-Text
Keywords: preeclampsia; soluble fms-like tyrosine kinase 1 (sFlt1); splice variants; isoforms; monoclonal antibody (mAb); diagnostic preeclampsia; soluble fms-like tyrosine kinase 1 (sFlt1); splice variants; isoforms; monoclonal antibody (mAb); diagnostic
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Souders, C.A.; Maynard, S.E.; Yan, J.; Wang, Y.; Boatright, N.K.; Sedan, J.; Balyozian, D.; Cheslock, P.S.; Molrine, D.C.; Simas, T.A.M. Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia. Int. J. Mol. Sci. 2015, 16, 12436-12453.

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