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Int. J. Mol. Sci. 2015, 16(5), 9976-9997; doi:10.3390/ijms16059976

Sasa quelpaertensis Leaf Extract Inhibits Colon Cancer by Regulating Cancer Cell Stemness in Vitro and in Vivo

1
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea
2
National Academy of Agricultural Science, Rural Development Administration, Jeollabuk-do 565-851, Korea
3
Department of Biology, Jeju National University, Jejusi, Jeju 690-756, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Sanjay K. Srivastava
Received: 6 March 2015 / Revised: 22 April 2015 / Accepted: 24 April 2015 / Published: 30 April 2015
(This article belongs to the Special Issue Bioactive Phytochemicals in Functional Foods for Cancer Prevention)
View Full-Text   |   Download PDF [2887 KB, uploaded 30 April 2015]   |  

Abstract

A rare subpopulation of cancer cells, termed cancer stem cells (CSCs), may be responsible for tumor relapse and resistance to conventional chemotherapy. The development of a non-toxic, natural treatment for the elimination of CSCs is considered a strategy for cancer treatment with minimal side effects. In the present study, the potential for Sasa quelpaertensis leaf extract (SQE) and its two bioactive compounds, tricin and p-coumaric acid, to exert anti-CSC effects by suppressing cancer stemness characteristics were evaluated in colon cancer cells. CD133+CD44+ cells were isolated from HT29 and HCT116 cell lines using flow-activated cell sorting (FACs). SQE treatment was found to significantly suppress the self-renewal capacity of both cell lines. SQE treatment was also associated with the down-regulation of β-catenin and phosphorylated GSK3β, while significantly enhancing cell differentiation by up-regulating CK20 expression and blocking the expression of several stem cell markers, including DLK1, Notch1, and Sox-2. In vivo, SQE supplementation suppressed tumor growth in a xenograft model by down-regulating stem cell markers and β-catenin as well as HIF-1α signaling. Compared with two bioactive compounds of SQE, SQE exhibited the most effective anti-CSC properties. Taken together, these results provide evidence that SQE inhibits colon cancer by regulating the characteristics of CSCs. View Full-Text
Keywords: Sasa quelpaertensis Nakai leaf extract; colon cancer; cancer stem cells; self-renewal; differentiation Sasa quelpaertensis Nakai leaf extract; colon cancer; cancer stem cells; self-renewal; differentiation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Min, S.J.; Lim, J.Y.; Kim, H.R.; Kim, S.-J.; Kim, Y. Sasa quelpaertensis Leaf Extract Inhibits Colon Cancer by Regulating Cancer Cell Stemness in Vitro and in Vivo. Int. J. Mol. Sci. 2015, 16, 9976-9997.

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