Next Article in Journal
Optimal Decision Rules for Biomarker-Based Subgroup Selection for a Targeted Therapy in Oncology
Next Article in Special Issue
Deep Sequencing Analysis of miRNA Expression in Breast Muscle of Fast-Growing and Slow-Growing Broilers
Previous Article in Journal
Cell Adhesion and in Vivo Osseointegration of Sandblasted/Acid Etched/Anodized Dental Implants
Previous Article in Special Issue
Identification of an AMPK Phosphorylation Site in Drosophila TSC2 (gigas) that Regulate Cell Growth
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(5), 10337-10353; doi:10.3390/ijms160510337

Effect of Melatonin on the Extracellular-Regulated Kinase Signal Pathway Activation and Human Osteoblastic Cell Line hFOB 1.19 Proliferation

1
Department of Orthopaedics, First Hospital, China Medical University, Shenyang 110001, China
2
Department of Orthopaedics, First Hospital, Dalian Medical University, Dalian 116011, China
3
Department of Orthopaedics, East Hospital, Tongji University School of Medicine, Shanghai 200120, China
*
Author to whom correspondence should be addressed.
Academic Editor: Alan C. Leonard
Received: 19 March 2015 / Revised: 21 April 2015 / Accepted: 27 April 2015 / Published: 7 May 2015
(This article belongs to the Special Issue Molecular Machinery of Cell Growth Regulation)
View Full-Text   |   Download PDF [1190 KB, uploaded 7 May 2015]   |  

Abstract

It has been shown that melatonin may affect bone metabolism. However, it is controversial whether melatonin could promote osteoblast proliferation, and the precise molecular mechanism of melatonin on osteoblast proliferation is still obscure. In this study, the results of the CCK-8 assay showed that melatonin significantly promoted human osteoblastic cell line hFOB 1.19 cell proliferation at 1, 2.5, 5, 10, 25, 50 and 100 µM concentrations for 24 h, but there were no significant differences among the groups. Western blot demonstrated that 10 µM melatonin significantly promoted ERK1/2 phosphorylation. Furthermore, we also detected the phosphorylation of c-Raf, MEK1/2, p90RSK and MSK1, and all of them increased with 10 µM melatonin. U0126 (a selective inhibitor of MEK that disrupts downstream activation of ERK1/2) downregulated the phosphorylation of ERK1/2, p90RSK and MSK1. U0126 also attenuated the proliferation of osteoblasts stimulated by melatonin. In conclusion, this study for the first time indicates that melatonin (10 nM–100 µM) promotes the proliferation of a human osteoblastic cell line hFOB 1.19 through activation of c-Raf, MEK1/2, ERK1/2, p90RSK and MSK1. View Full-Text
Keywords: melatonin; osteoblasts; proliferation; extracellular signal-regulated kinase; signal transduction melatonin; osteoblasts; proliferation; extracellular signal-regulated kinase; signal transduction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Xiong, X.-C.; Zhu, Y.; Ge, R.; Liu, L.-F.; Yuan, W. Effect of Melatonin on the Extracellular-Regulated Kinase Signal Pathway Activation and Human Osteoblastic Cell Line hFOB 1.19 Proliferation. Int. J. Mol. Sci. 2015, 16, 10337-10353.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top