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Int. J. Mol. Sci. 2015, 16(4), 6945-6959; doi:10.3390/ijms16046945

Effects of Aflatoxin B1 on T-Cell Subsets and mRNA Expression of Cytokines in the Intestine of Broilers

1,†
,
1,2,†
,
1,2,* , 1,2
,
1
and
2
1
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya’an 625014, Sichuan, China
2
College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, Sichuan, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Hansruedi Glatt
Received: 18 January 2015 / Revised: 9 March 2015 / Accepted: 19 March 2015 / Published: 27 March 2015
(This article belongs to the Section Molecular Toxicology)
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Abstract

This study was conducted to investigate the effects of aflatoxin B1 (AFB1) on T-cell subsets and mRNA expression of cytokines in the small intestine of broilers. One hundred and fifty-six one-day-old healthy Cobb broilers were randomly divided into control group (0 mg/kg AFB1) and AFB1 group (0.6 mg/kg AFB1) with three replicates per group and 26 birds per replicate for 21 days, respectively. At 7, 14, and 21 days of age, the duodenum, jejunum and ileum were sampled for analyzing T cell subsets (CD3+, CD3+CD4+ and CD3+CD8+) by flow cytometry as well as IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α mRNA expression by qRT-PCR. The percentages of T-cells in the intra-epithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) of duodenum, jejunum and ileum in the AFB1 group showed a decreased tendency in comparison to the control group. The mRNA expression of cytokines in the three intestinal segments in the AFB1 group presented a general decline compared with the control groups. Our data demonstrated that 0.6 mg/kg AFB1 in the broilers diet could reduce the percentages of T-cell subsets and the expression level of cytokine mRNA in the small intestine, implying that the immune function of the intestinal mucosa might be affected. The reduction of cytokines mRNA expression may be closely associated with the decreased proportions of T cells subsets induced by AFB1. View Full-Text
Keywords: aflatoxin B1; T-cell subsets; cytokine; intestinal mucosal immunity; broilers aflatoxin B1; T-cell subsets; cytokine; intestinal mucosal immunity; broilers
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jiang, M.; Peng, X.; Fang, J.; Cui, H.; Yu, Z.; Chen, Z. Effects of Aflatoxin B1 on T-Cell Subsets and mRNA Expression of Cytokines in the Intestine of Broilers. Int. J. Mol. Sci. 2015, 16, 6945-6959.

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