Next Article in Journal
Nano SiO2 and MgO Improve the Properties of Porous β-TCP Scaffolds via Advanced Manufacturing Technology
Next Article in Special Issue
A Review of the Effect of Diet on Cardiovascular Calcification
Previous Article in Journal
Towards Understanding the Decomposition/Isomerism Channels of Stratospheric Bromine Species: Ab Initio and Quantum Topology Study
Previous Article in Special Issue
SOD2 Activity Is not Impacted by Hyperoxia in Murine Neonatal Pulmonary Artery Smooth Muscle Cells and Mice
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(4), 6801-6817; doi:10.3390/ijms16046801

Preservation of Renal Blood Flow by the Antioxidant EUK-134 in LPS-Treated Pigs

1
Critical Care Division, Royal Victoria Hospital, 687 Pine Ave W, Montreal, QC H3A 1A1, Canada
2
Vascular Surgery of Nikaia, Peiraeous 18454, Greece
3
Vascular Medicine Institute and Department of Pharmacology and Chemical Biology, University of Pittsburgh, Room E1228-2B, 200 Lothrop St, Pittsburgh, PA 15261, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Francis J. Miller
Received: 4 November 2014 / Accepted: 6 March 2015 / Published: 25 March 2015
(This article belongs to the Special Issue Oxidative Stress in Cardiovascular Disease 2015)
View Full-Text   |   Download PDF [854 KB, uploaded 1 April 2015]   |  

Abstract

Sepsis is associated with an increase in reactive oxygen species (ROS), however, the precise role of ROS in the septic process remains unknown. We hypothesized that treatment with EUK-134 (manganese-3-methoxy N,N'-bis(salicyclidene)ethylene-diamine chloride), a compound with superoxide dismutase and catalase activity, attenuates the vascular manifestations of sepsis in vivo. Pigs were instrumented to measure cardiac output and blood flow in renal, superior mesenteric and femoral arteries, and portal vein. Animals were treated with saline (control), lipopolysaccharide (LPS; 10 µg·kg−1·h−1), EUK-134, or EUK-134 plus LPS. Results show that an LPS-induced increase in pulmonary artery pressure (PAP) as well as a trend towards lower blood pressure (BP) were both attenuated by EUK-134. Renal blood flow decreased with LPS whereas superior mesenteric, portal and femoral flows did not change. Importantly, EUK-134 decreased the LPS-induced fall in renal blood flow and this was associated with a corresponding decrease in LPS-induced protein nitrotyrosinylation in the kidney. PO2, pH, base excess and systemic vascular resistance fell with LPS and were unaltered by EUK-134. EUK-134 also had no effect on LPS-associated increase in CO. Interestingly, EUK-134 alone resulted in higher CO, BP, PAP, mean circulatory filling pressure, and portal flow than controls. Taken together, these data support a protective role for EUK-134 in the renal circulation in sepsis. View Full-Text
Keywords: endotoxin; sepsis; superoxide dismutase; catalase; peroxynitrite; EUK-134 endotoxin; sepsis; superoxide dismutase; catalase; peroxynitrite; EUK-134
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Magder, S.; Parthenis, D.G.; Ghouleh, I.A. Preservation of Renal Blood Flow by the Antioxidant EUK-134 in LPS-Treated Pigs. Int. J. Mol. Sci. 2015, 16, 6801-6817.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top