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Int. J. Mol. Sci. 2015, 16(3), 6235-6250; doi:10.3390/ijms16036235

Abortive Infection of Snakehead Fish Vesiculovirus in ZF4 Cells Was Associated with the RLRs Pathway Activation by Viral Replicative Intermediates

1
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
2
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China
3
Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), El-Kanater 13621, Egypt
4
School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China
5
Wuhan Fishery Research Institute, Wuhan 430207, China
6
Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 14 February 2015 / Revised: 7 March 2015 / Accepted: 10 March 2015 / Published: 18 March 2015
(This article belongs to the Special Issue Fish Molecular Biology)
View Full-Text   |   Download PDF [854 KB, uploaded 18 March 2015]   |  

Abstract

Snakehead fish vesiculovirus (SHVV) is a negative strand RNA virus which can cause great economic losses in fish culture. To facilitate the study of SHVV-host interactions, the susceptibility of zebrafish embryonic fibroblast cell line (ZF4) to the SHVV was investigated in this report. The results showed that high amount of viral mRNAs and cRNAs were detected at the 3 h post-infection. However, the expressions of the viral mRNAs and cRNA were decreased dramatically after 6 h post-infection. In addition, the expressions of interferon (IFN) and interferon-induced GTP-binding protein Mx were all up regulated significantly at the late stage of the infection. Meanwhile, the expressions of Retinoic acid-inducible gene I (RIG-I) and Melanoma differentiation-associated gene 5 (MDA5) were also all up-regulated significantly during the infection. Two isoforms of DrLGP2 from zebrafish were also cloned and analyzed. Interestingly, the expression of DrLGP2a but not DrLGP2b was significantly up-regulated at both mRNA and protein levels, indicating that the two DrLGP2 isoforms might play different roles during the SHVV infection. Transfection experiment showed that viral replicative intermediates were required for the activation of IFN-α expression. Taken together, the abortive infection of SHVV in ZF4 cells was associated with the activation of RLRs pathway, which was activated by viral replicative intermediates. View Full-Text
Keywords: snakehead fish vesiculovirus; ZF4; infection; Retinoic acid-inducible gene I (RIG-I)-like receptors; interferon; viral replicative intermediates snakehead fish vesiculovirus; ZF4; infection; Retinoic acid-inducible gene I (RIG-I)-like receptors; interferon; viral replicative intermediates
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, W.; Asim, M.; Yi, L.; Hegazy, A.M.; Hu, X.; Zhou, Y.; Ai, T.; Lin, L. Abortive Infection of Snakehead Fish Vesiculovirus in ZF4 Cells Was Associated with the RLRs Pathway Activation by Viral Replicative Intermediates. Int. J. Mol. Sci. 2015, 16, 6235-6250.

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