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Int. J. Mol. Sci. 2015, 16(3), 6057-6075; doi:10.3390/ijms16036057

Protease and Hemicellulase Assisted Extraction of Dietary Fiber from Wastes of Cynara cardunculus

1
Industry Department, School of Natural and Exact Sciences, Buenos Aires University (UBA), Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina
2
Fellow of the National Research Council of Argentina (CONICET), 1033 Ciudad Autónoma de Buenos Aires, Argentina
3
Instituto de Investigaciones en Biociencias Agrícolas y Ambientales—INBA (CONICET), School of Agronomy (UBA), 1417 Ciudad Autónoma de Buenos Aires, Argentina
4
Member of CONICET, 1033 Ciudad Autónoma de Buenos Aires, Argentina
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas J. Farmer
Received: 4 February 2015 / Revised: 3 March 2015 / Accepted: 10 March 2015 / Published: 16 March 2015
(This article belongs to the Special Issue Green Chemistry and the Biorefinery)
View Full-Text   |   Download PDF [740 KB, uploaded 16 March 2015]   |  

Abstract

The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0–200 μL and hemicellulase amounts of 0–200 mg for 5 g of material, we explored the effect of a 5 h enzymatic treatment at 40 °C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol. View Full-Text
Keywords: Cynara cardunculus; protease; hemicellulase; biomass to chemicals; inulin; pectin; polyphenols Cynara cardunculus; protease; hemicellulase; biomass to chemicals; inulin; pectin; polyphenols
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Domingo, C.S.; Soria, M.; Rojas, A.M.; Fissore, E.N.; Gerschenson, L.N. Protease and Hemicellulase Assisted Extraction of Dietary Fiber from Wastes of Cynara cardunculus. Int. J. Mol. Sci. 2015, 16, 6057-6075.

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