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Int. J. Mol. Sci. 2015, 16(3), 4904-4917; doi:10.3390/ijms16034904

Secretion of Human Protein C in Mouse Milk

1
Animal Biotechnology, Graduate School of Bio and Information Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea
2
Department of Food and Nutrition, Hoseo University, Asan 336-795, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Mark L. Richter
Received: 24 December 2014 / Revised: 10 February 2015 / Accepted: 26 February 2015 / Published: 4 March 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

To determine the production of recombinant human protein C (rec-hPC) in milk, we created two homozygous mice lines for the goat β-casein/hPC transgene. Females and males of both lines (#10 and #11) displayed normal growth, fertility, and lactated normally. The copy number of the transgene was about fivefold higher in #10 line as compared to #11 line. mRNA expression of the transgene was only detected in the mammary glands of both lines. Furthermore, mRNA expression was fourfold higher on day 7 than on day 1 during lactation. Northern blot analysis of mRNA expression in the #10 line of transgenic (Tg) mice indicated a strong expression of the transgene in the mammary glands after seven days of lactation. Comparison of rec-hPC protein level with that of mRNA in the mammary glands showed a very similar pattern. A 52-kDa band corresponding to the hPC protein was strongly detected in mammary glands of the #10 line during lactation. We also detected two bands of heavy chain and one weak band of light chain in the milk of the #10 and #11 lines. One single band at 52 kDa was detected from CHO cells transfected with hPC cDNA. hPC was mainly localized in the alveolar epithelial cell of the mammary glands. The protein is strongly expressed in the cytoplasm of the cultured mammary gland tissue. hPC protein produced in milk ranged from 2 to 28 ng/mL. These experiments indicated that rec-hPC can be produced at high levels in mice mammary glands. View Full-Text
Keywords: human protein C; transgenic mice; mammary glands human protein C; transgenic mice; mammary glands
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Park, C.-W.; Kang, M.-H.; Min, K.-S. Secretion of Human Protein C in Mouse Milk. Int. J. Mol. Sci. 2015, 16, 4904-4917.

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