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Int. J. Mol. Sci. 2015, 16(2), 4265-4280; doi:10.3390/ijms16024265

β-Hydroxybutyric Sodium Salt Inhibition of Growth Hormone and Prolactin Secretion via the cAMP/PKA/CREB and AMPK Signaling Pathways in Dairy Cow Anterior Pituitary Cells

1,2,†
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1,†
,
1,†
,
2,†
,
2,†
,
1
,
1
,
1,* and 1,2,*
1
College of Veterinary Medicine, Jilin University, Changchun 130062, China
2
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Kathleen Van Craenenbroeck
Received: 17 November 2014 / Revised: 16 January 2015 / Accepted: 9 February 2015 / Published: 16 February 2015
(This article belongs to the Collection G Protein-Coupled Receptor Signaling and Regulation)
View Full-Text   |   Download PDF [2267 KB, uploaded 16 February 2015]   |  

Abstract

β-hydroxybutyric acid (BHBA) regulates the synthesis and secretion of growth hormone (GH) and prolactin (PRL), but its mechanism is unknown. In this study, we detected the effects of BHBA on the activities of G protein signaling pathways, AMPK-α activity, GH, and PRL gene transcription, and GH and PRL secretion in dairy cow anterior pituitary cells (DCAPCs). The results showed that BHBA decreased intracellular cAMP levels and a subsequent reduction in protein kinase A (PKA) activity. Inhibition of PKA activity reduced cAMP response element-binding protein (CREB) phosphorylation, thereby inhibiting GH and PRL transcription and secretion. The effects of BHBA were attenuated by a specific Gαi inhibitor, pertussis toxin (PTX). In addition, intracellular BHBA uptake mediated by monocarboxylate transporter 1 (MCT1) could trigger AMPK signaling and result in the decrease in GH and PRL mRNA translation in DCAPCs cultured under low-glucose and non-glucose condition when compared with the high-glucose group. This study identifies a biochemical mechanism for the regulatory action of BHBA on GH and PRL gene transcription, translation, and secretion in DCAPCs, which may be one of the factors that regulate pituitary function during the transition period in dairy cows. View Full-Text
Keywords: β-hydroxybutyric acid; dairy cow anterior pituitary cells; growth hormone; prolactin β-hydroxybutyric acid; dairy cow anterior pituitary cells; growth hormone; prolactin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Fu, S.-P.; Wang, W.; Liu, B.-R.; Yang, H.-M.; Ji, H.; Yang, Z.-Q.; Guo, B.; Liu, J.-X.; Wang, J.-F. β-Hydroxybutyric Sodium Salt Inhibition of Growth Hormone and Prolactin Secretion via the cAMP/PKA/CREB and AMPK Signaling Pathways in Dairy Cow Anterior Pituitary Cells. Int. J. Mol. Sci. 2015, 16, 4265-4280.

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