Next Article in Journal
The Association between Polymorphism of INSR and Polycystic Ovary Syndrome: A Meta-Analysis
Previous Article in Journal
Oxidative Stress, Bone Marrow Failure, and Genome Instability in Hematopoietic Stem Cells
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(2), 2386-2402; doi:10.3390/ijms16022386

Quantitative Expression Analysis of APP Pathway and Tau Phosphorylation-Related Genes in the ICV STZ-Induced Non-Human Primate Model of Sporadic Alzheimer’s Disease

1
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Korea
2
University of Science & Technology, National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Korea
3
Graduate School Department of Digital Media, Ewha Womans University, Seoul 120-750, Korea
4
Department of Neurology, Seoul National University Hospital, Seoul 110-744, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Kurt A. Jellinger
Received: 4 November 2014 / Revised: 14 January 2015 / Accepted: 16 January 2015 / Published: 22 January 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1276 KB, uploaded 22 January 2015]   |  

Abstract

The accumulation and aggregation of misfolded proteins in the brain, such as amyloid-β (Aβ) and hyperphosphorylated tau, is a neuropathological hallmark of Alzheimer’s disease (AD). Previously, we developed and validated a novel non-human primate model for sporadic AD (sAD) research using intracerebroventricular administration of streptozotocin (icv STZ). To date, no characterization of AD-related genes in different brain regions has been performed. Therefore, in the current study, the expression of seven amyloid precursor protein (APP) pathway-related and five tau phosphorylation-related genes was investigated by quantitative real-time PCR experiments, using two matched-pair brain samples from control and icv STZ-treated cynomolgus monkeys. The genes showed similar expression patterns within the control and icv STZ-treated groups; however, marked differences in gene expression patterns were observed between the control and icv STZ-treated groups. Remarkably, other than β-secretase (BACE1) and cyclin-dependent kinase 5 (CDK5), all the genes tested showed similar expression patterns in AD models compared to controls, with increased levels in the precuneus and occipital cortex. However, significant changes in gene expression patterns were not detected in the frontal cortex, hippocampus, or posterior cingulate. Based on these results, we conclude that APP may be cleaved via the general metabolic mechanisms of increased α- and γ-secretase levels, and that hyperphosphorylation of tau could be mediated by elevated levels of tau protein kinase, specifically in the precuneus and occipital cortex. View Full-Text
Keywords: Alzheimer’s disease; streptosozocin; cynomolgus monkey; qPCR; APP; tau Alzheimer’s disease; streptosozocin; cynomolgus monkey; qPCR; APP; tau
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Park, S.-J.; Kim, Y.-H.; Nam, G.-H.; Choe, S.-H.; Lee, S.-R.; Kim, S.-U.; Kim, J.-S.; Sim, B.-W.; Song, B.-S.; Jeong, K.-J.; Lee, Y.; Park, Y.I.; Lee, K.-M.; Huh, J.-W.; Chang, K.-T. Quantitative Expression Analysis of APP Pathway and Tau Phosphorylation-Related Genes in the ICV STZ-Induced Non-Human Primate Model of Sporadic Alzheimer’s Disease. Int. J. Mol. Sci. 2015, 16, 2386-2402.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top