Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells
AbstractTudor staphylococcal nuclease (Tudor-SN) is a highly conserved and ubiquitously expressed multifunctional protein, related to multiple and diverse cell type- and species-specific cellular processes. Studies have shown that Tudor-SN is mainly expressed in secretory cells, however knowledge of its role is limited. In our previous work, we found that the protein level of Tudor-SN was upregulated in the nucleus of bovine mammary epithelial cells (BMEC). In this study, we assessed the role of Tudor-SN in milk synthesis and cell proliferation of BMEC. We exploited gene overexpression and silencing methods, and found that Tudor-SN positively regulates milk synthesis and proliferation via Stat5a activation. Both amino acids (methionine) and estrogen triggered NFκB1 to bind to the gene promoters of Tudor-SN and Stat5a, and this enhanced the protein level and nuclear localization of Tudor-SN and p-Stat5a. Taken together, these results suggest the key role of Tudor-SN in the transcriptional regulation of milk synthesis and proliferation of BMEC under the stimulation of amino acids and hormones. View Full-Text
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Ao, J.; Wei, C.; Si, Y.; Luo, C.; Lv, W.; Lin, Y.; Cui, Y.; Gao, X. Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells. Int. J. Mol. Sci. 2015, 16, 29936-29947.
Ao J, Wei C, Si Y, Luo C, Lv W, Lin Y, Cui Y, Gao X. Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells. International Journal of Molecular Sciences. 2015; 16(12):29936-29947.Chicago/Turabian Style
Ao, Jinxia; Wei, Chengjie; Si, Yu; Luo, Chaochao; Lv, Wei; Lin, Ye; Cui, Yingjun; Gao, Xuejun. 2015. "Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells." Int. J. Mol. Sci. 16, no. 12: 29936-29947.