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Int. J. Mol. Sci. 2015, 16(12), 29787-29796; doi:10.3390/ijms161226134

Identification of Novel Chromosomal Aberrations Induced by 60Co-γ-Irradiation in Wheat-Dasypyrum villosum Lines

1
Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 610061, Sichuan, China
2
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
3
Department of Cell Biology and Genetics, Zunyi Medical University, Zunyi 563000, Guizhou, China
4
Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China
*
Author to whom correspondence should be addressed.
Academic Editor: Dilantha Fernando
Received: 15 October 2015 / Revised: 20 November 2015 / Accepted: 25 November 2015 / Published: 14 December 2015
(This article belongs to the Section Molecular Toxicology)
View Full-Text   |   Download PDF [5068 KB, uploaded 14 December 2015]   |  

Abstract

Mutations induced by radiation are widely used for developing new varieties of plants. To better understand the frequency and pattern of irradiation-induced chromosomal rearrangements, we irradiated the dry seeds of Chinese Spring (CS)-Dasypyrum villosum nullisomic-tetrasomic (6A/6D) addition (6V) line (2n = 44), WD14, with 60Co-γ-rays at dosages of 100, 200, and 300 Gy. The M0 and M1 generations were analyzed using Feulgen staining and non-denaturing fluorescence in situ hybridization (ND-FISH) by using oligonucleotide probes. Abnormal mitotic behavior and chromosomes with structural changes were observed in the M0 plants. In all, 39 M1 plants had structurally changed chromosomes, with the B genome showing the highest frequency of aberrations and tendency to recombine with chromosomes of the D genome. In addition, 19 M1 plants showed a variation in chromosome number. The frequency of chromosome loss was considerably higher for 6D than for the alien chromosome 6V, indicating that 6D is less stable after irradiation. Our findings suggested that the newly obtained γ-induced genetic materials might be beneficial for future wheat breeding programs and functional gene analyses. View Full-Text
Keywords: irradiation; wheat; Dasypyrum villosum; chromosomal aberrations; ND-FISH irradiation; wheat; Dasypyrum villosum; chromosomal aberrations; ND-FISH
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, J.; Jiang, Y.; Guo, Y.; Li, G.; Yang, Z.; Xu, D.; Xuan, P. Identification of Novel Chromosomal Aberrations Induced by 60Co-γ-Irradiation in Wheat-Dasypyrum villosum Lines. Int. J. Mol. Sci. 2015, 16, 29787-29796.

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