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Int. J. Mol. Sci. 2015, 16(12), 27988-28000; doi:10.3390/ijms161226081

Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

1
Department of Oral Implantology, Osaka Dental University, Osaka 573-1121, Japan
2
Institute of Dental Research, Osaka Dental University, Osaka 573-1121, Japan
3
Department of Biomaterials, Osaka Dental University, Osaka 573-1121, Japan
4
Department of Fixed Prosthodontics and Occlusion, Osaka Dental University, Osaka 573-1121, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Paula Andrade
Received: 11 September 2015 / Revised: 16 November 2015 / Accepted: 18 November 2015 / Published: 25 November 2015
(This article belongs to the Special Issue Phenolics and Polyphenolics 2015)
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Abstract

Despite advances in stem cell biology, there are few effective techniques to promote the osteogenic differentiation of human primary dedifferentiated fat (DFAT) cells. We attempted to investigate whether epigallocatechin-3-gallate (EGCG), the main component of green tea catechin, facilitates early osteogenic differentiation and mineralization on DFAT cells in vitro. DFAT cells were treated with EGCG (1.25–10 μM) in osteogenic medium (OM) with or without 100 nM dexamethasone (Dex) for 12 days (hereafter two osteogenic media were designated as OM(Dex) and OM). Supplementation of 1.25 μM EGCG to both the media effectively increased the mRNA expression of collagen 1 (COL1A1) and runt-related transcription factor 2 (RUNX2) and also increased proliferation and mineralization. Compared to OM(Dex) with EGCG, OM with EGCG induced earlier expression for COL1A1 and RUNX2 at day 1 and higher mineralization level at day 12. OM(Dex) with 10 μM EGCG remarkably hampered the proliferation of the DFAT cells. These results suggest that OM(without Dex) with EGCG might be a preferable medium to promote proliferation and to induce osteoblast differentiation of DFAT cells. Our findings provide an insight for the combinatory use of EGCG and DFAT cells for bone regeneration and stem cell-based therapy. View Full-Text
Keywords: polyphenol; catechin; EGCG; bone; dedifferentiated fat cell; stem cells; osteoblast polyphenol; catechin; EGCG; bone; dedifferentiated fat cell; stem cells; osteoblast
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Kaida, K.; Honda, Y.; Hashimoto, Y.; Tanaka, M.; Baba, S. Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro. Int. J. Mol. Sci. 2015, 16, 27988-28000.

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