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Int. J. Mol. Sci. 2015, 16(11), 27589-27598; doi:10.3390/ijms161126048

Brazilein Suppresses Inflammation through Inactivation of IRAK4-NF-κB Pathway in LPS-Induced Raw264.7 Macrophage Cells

1
Department of Food Science and Biotechnology, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-300, Korea
2
Department of Biomedical Laboratory Science, Soon Chun Hyang University, Asan, Chungnam 336-745, Korea
3
Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungbuk 363-883, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Antonio Segura-Carretero
Received: 29 September 2015 / Revised: 10 November 2015 / Accepted: 10 November 2015 / Published: 18 November 2015
(This article belongs to the Special Issue Advances in Molecular Research of Functional and Nutraceutical Food)
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Abstract

The medicinal herbal plant has been commonly used for prevention and intervention of disease and health promotions worldwide. Brazilein is a bioactive compound extracted from Caesalpinia sappan Linn. Several studies have showed that brazilein exhibited the immune suppressive effect and anti-oxidative function. However, the molecular targets of brazilein for inflammation prevention have remained elusive. Here, we investigated the mechanism underlying the inhibitory effect of brazilein on LPS-induced inflammatory response in Raw264.7 macrophage cells. We demonstrated that brazilein decreased the expression of IRAK4 protein led to the suppression of MAPK signaling and IKKβ, and subsequent inactivation of NF-κB and COX2 thus promoting the expression of the downstream target pro-inflammatory cytokines such as IL-1β, MCP-1, MIP-2, and IL-6 in LPS-induced Raw264.7 macrophage cells. Moreover, we observed that brazilein reduced the production of nitrite compared to the control in LPS-induced Raw264.7. Thus, we suggest that brazilein might be a useful bioactive compound for the prevention of IRAK-NF-κB pathway associated chronic diseases. View Full-Text
Keywords: brazilein; inflammation; functional food; health benefits; NF-κB; toll-like receptors; MAPK; IRAK4 brazilein; inflammation; functional food; health benefits; NF-κB; toll-like receptors; MAPK; IRAK4
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kim, K.-J.; Yoon, K.-Y.; Yoon, H.-S.; Oh, S.-R.; Lee, B.-Y. Brazilein Suppresses Inflammation through Inactivation of IRAK4-NF-κB Pathway in LPS-Induced Raw264.7 Macrophage Cells. Int. J. Mol. Sci. 2015, 16, 27589-27598.

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