Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies
AbstractIn this review, we disclose a selection of serum/plasma biomarkers of (anti)oxidant status related to nutrition, which can be used for measurements in large-scale epidemiological studies. From personal experience, we have come to the following proposal of a set of biomarkers for nutritional intake, (anti)oxidant status, and redox status. We have selected the individual antioxidant vitamins E and A, and the carotenoids which can be measured in large series by HPLC. In addition, vitamin C was selected, which can be measured by an auto-analyzer or HPLC. As a biomarker for oxidative stress, the ROM assay (reactive oxygen metabolites) was selected; for the redox status, the total thiol assay; and for the total antioxidant status the BAP assay (biological antioxidant potential). All of these biomarkers can be measured in large quantities by an auto-analyzer. Critical points in biomarker validation with respect to blood sampling, storage conditions, and measurements are discussed. With the selected biomarkers, a good set is presented for use in the risk assessment between nutrition and (chronic) diseases in large-scale epidemiological studies. Examples of the successful application of these biomarkers in large international studies are presented. View Full-Text
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Jansen, E.; Ruskovska, T. Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies. Int. J. Mol. Sci. 2015, 16, 27378-27390.
Jansen E, Ruskovska T. Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies. International Journal of Molecular Sciences. 2015; 16(11):27378-27390.Chicago/Turabian Style
Jansen, Eugène; Ruskovska, Tatjana. 2015. "Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies." Int. J. Mol. Sci. 16, no. 11: 27378-27390.