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Int. J. Mol. Sci. 2015, 16(11), 27208-27227; doi:10.3390/ijms161126023

Developing Potential Candidates of Preclinical Preeclampsia

1
School of Nursing and Magee-Womens Research Institute, University of Pittsburgh, 3500 Victoria St. 448 VB, Pittsburgh, PA 15261, USA
2
Biomedical Mass Spectrometry Center Schools of the Health Sciences, University of Pittsburgh, Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, PA 15261, USA
3
Magee-Womens Research Institute, University of Pittsburgh, 204 Craft Avenue Pittsburgh, PA 15213, USA
4
School of Medicine, Graduate School of Public Health and Magee-Womens Research Institute, University of Pittsburgh, 204 Craft Avenue Pittsburgh, PA 15213, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Philip Newton Baker
Received: 25 August 2015 / Revised: 28 October 2015 / Accepted: 3 November 2015 / Published: 13 November 2015
(This article belongs to the Special Issue Prediction, Diagnostics and Prevention of Pregnancy Complications)
View Full-Text   |   Download PDF [673 KB, uploaded 13 November 2015]   |  

Abstract

The potential for developing molecules of interest in preclinical preeclampsia from candidate genes that were discovered on gene expression microarray analysis has been challenged by limited access to additional first trimester trophoblast and decidual tissues. The question of whether these candidates encode secreted proteins that may be detected in maternal circulation early in pregnancy has been investigated using various proteomic methods. Pilot studies utilizing mass spectrometry based proteomic assays, along with enzyme linked immunosorbent assays (ELISAs), and Western immunoblotting in first trimester samples are reported. The novel targeted mass spectrometry methods led to robust multiple reaction monitoring assays. Despite detection of several candidates in early gestation, challenges persist. Future antibody-based studies may lead to a novel multiplex protein panel for screening or detection to prevent or mitigate preeclampsia. View Full-Text
Keywords: preeclampsia; candidate gene; candidate protein; biomarker development; prevention preeclampsia; candidate gene; candidate protein; biomarker development; prevention
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Founds, S.; Zeng, X.; Lykins, D.; Roberts, J.M. Developing Potential Candidates of Preclinical Preeclampsia. Int. J. Mol. Sci. 2015, 16, 27208-27227.

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