Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats
Abstract
:1. Introduction
2. Results and Discussion
2.1. CAPN3 Is Expressed in the Gastrocnemius Muscle Following Sciatic Nerve Crush Injury
2.2. CAPN3 Expression Is Associated with Gastrocnemius Muscle Atrophy
2.3. CAPN3 Deficiency Promotes L6 Cell Differentiation and Delays Skeletal Muscle Atrophy
3. Experimental Section
3.1. Animals and Surgical Procedures
3.2. Functional and Morphometric Analyses Following Sciatic Nerve Crush Injury
3.3. CAPN3 Short Interfering (si) RNA Knockdown
Usage | Target | Sequence (5′ to 3′) |
---|---|---|
qRT-PCR | GAPDH sense | CCTTCATTGACCTCAACTACATG |
GAPDH antisense | TCAAACTTGTGATCCAGGCG | |
CAPN3 sense | CATTGTCCCCTCCACTTACG | |
CAPN3 antisense | GCTCCTTGTTGCTGTTTGC | |
MyoD sense | CGACTCTTCAGGCTTGGGTT | |
MyoD antisense | CCAGGTCCTCAAAAAAGCG | |
siRNA sequence | Ctrl sense | CCUACGCCACCAAUUUCGUTT |
Ctrl antisense | ACGAAAUUGGUGGCGUAGGTT | |
CAPN3 sense | CUGAAGACAAGGGUUCATT | |
CAPN3 antisense | UGAACCCUUGUGUCUUCAGTT |
3.4. Real-Time (RT-) PCR and Western Blot Analysis
3.5. L6 Cell Culture, Differentiation and Myotube Calculation
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wu, R.; Yan, Y.; Yao, J.; Liu, Y.; Zhao, J.; Liu, M. Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats. Int. J. Mol. Sci. 2015, 16, 26927-26935. https://doi.org/10.3390/ijms161126003
Wu R, Yan Y, Yao J, Liu Y, Zhao J, Liu M. Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats. International Journal of Molecular Sciences. 2015; 16(11):26927-26935. https://doi.org/10.3390/ijms161126003
Chicago/Turabian StyleWu, Ronghua, Yingying Yan, Jian Yao, Yan Liu, Jianmei Zhao, and Mei Liu. 2015. "Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats" International Journal of Molecular Sciences 16, no. 11: 26927-26935. https://doi.org/10.3390/ijms161126003