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Int. J. Mol. Sci. 2015, 16(10), 25759-25772; doi:10.3390/ijms161025759

Identification and Analysis of Regulatory Elements in Porcine Bone Morphogenetic Protein 15 Gene Promoter

Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University, Xi’an 712100, Shaanxi, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Xiaofeng Jia
Received: 6 August 2015 / Revised: 16 October 2015 / Accepted: 20 October 2015 / Published: 27 October 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Bone morphogenetic protein 15 (BMP15) is secreted by the mammalian oocytes and is indispensable for ovarian follicular development, ovulation, and fertility. To determine the regulation mechanism of BMP15 gene, the regulatory sequence of porcine BMP15 was investigated in this study. The cloned BMP15 promoter retains the cell-type specificity, and is activated in cells derived from ovarian tissue. The luciferase assays in combination with a series of deletion of BMP15 promoter sequence show that the −427 to −376 bp region of BMP15 promoter is the primary regulatory element, in which there are a number of transcription factor binding sites, including LIM homeobox 8 (LHX8), newborn ovary homeobox gene (NOBOX), and paired-like homeodomain transcription factor 1 (PITX1). Determination of tissue-specific expression reveals that LHX8, but not PITX1 and NOBOX, is exclusively expressed in pig ovary tissue and is translocated into the cell nuclei. Overexpression of LHX8 in Chinese hamster ovary (CHO) cells could significantly promote BMP15 promoter activation. This study confirms a key regulatory element that is located in the proximal region of BMP15 promoter and is regulated by the LHX8 factor. View Full-Text
Keywords: pig; BMP15; promoter; transcription factor; LHX8 pig; BMP15; promoter; transcription factor; LHX8
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wan, Q.; Wang, Y.; Wang, H. Identification and Analysis of Regulatory Elements in Porcine Bone Morphogenetic Protein 15 Gene Promoter. Int. J. Mol. Sci. 2015, 16, 25759-25772.

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