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Int. J. Mol. Sci. 2015, 16(10), 24302-24318; doi:10.3390/ijms161024302

Identification and Functional Analysis of MicroRNAs in Mice following Focal Cerebral Ischemia Injury

1,2,3,†
,
4,†
,
2
,
2,* and 1,2,*
1
Institute of Biomedical Engineering, Capital Medical University, Beijing 100069, China
2
Department of Neurobiology and Center of Stroke, Beijing Institute for Brain Disorders, Capital Medical University, Beijing 100069, China
3
China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China
4
Department of Anesthesiology Xuan Wu Hospital, Capital Medical University, Beijing 100053, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Y-h. Taguchi
Received: 6 August 2015 / Revised: 9 September 2015 / Accepted: 10 September 2015 / Published: 14 October 2015
(This article belongs to the Special Issue MicroRNA Regulation)
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Abstract

Numerous studies have demonstrated that genes, RNAs, and proteins are involved in the occurrence and development of stroke. In addition, previous studies concluded that microRNAs (miRNAs or miRs) are closely related to the pathological process of ischemic and hypoxic disease. Therefore, the aims of this study were to quantify the altered expression levels of miRNAs in the infarct region 6 h after middle cerebral artery occlusion (MCAO)-induced focal cerebral ischemia in mice using a large-scale miRNAs microarray. Firstly, MCAO-induced cerebral ischemic injuries were investigated by observing the changes of neurological deficits, infarct volume and edema ratio. One hundred and eighteen differentially expressed miRNAs were identified in the infarct region of mice following the MCAOs compared with sham group (p < 0.05 was considered as significant). Among these 118 significantly expressed microRNAs, we found that 12 miRNAs were up-regulated with fold changes lager than two, and 18 miRNAs were down-regulated with fold changes less than 0.5 in the infarct region of mice following the 6 h MCAOs, compared with the sham group. Then, these 30 miRNAs with expression in fold change larger than two or less than 0.5 was predicted, and the functions of the target genes of 30 miRNAs were analyzed using a bioinformatics method. Finally, the miRNA-gene network was established and the functional miRNA-mRNA pairs were identified, which provided insight into the roles of the specific miRNAs that regulated specified genes in the ischemic injuries. The miRNAs identified in this study may represent effective therapeutic targets for stroke, and further study of the role of these targets may increase our understanding of the mechanisms underlying ischemic injuries. View Full-Text
Keywords: middle cerebral artery occlusion; miRNA microarray; gene ontology; Kyoto encyclopedia of genes and genomes; network middle cerebral artery occlusion; miRNA microarray; gene ontology; Kyoto encyclopedia of genes and genomes; network
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MDPI and ACS Style

Liu, C.; Zhao, L.; Han, S.; Li, J.; Li, D. Identification and Functional Analysis of MicroRNAs in Mice following Focal Cerebral Ischemia Injury. Int. J. Mol. Sci. 2015, 16, 24302-24318.

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