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Int. J. Mol. Sci. 2015, 16(10), 23250-23258; doi:10.3390/ijms161023250

Secreted Frizzled-Related Protein Promotes Bone Regeneration by Human Bone Marrow-Derived Mesenchymal Stem Cells

Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan
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Academic Editor: Wilhelm Bloch
Received: 4 August 2015 / Revised: 10 September 2015 / Accepted: 23 September 2015 / Published: 25 September 2015
(This article belongs to the Special Issue Stem Cell Activation in Adult Organism)
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Abstract

Secreted frizzled-related protein (sFRP)-3 is a negative regulator of Wnt signaling in human mesenchymal stem cells (hMSCs). The present study investigated the effects sFRP-3 on osteogenic differentiation by assessing osteogenic gene expression in hMSCs in vitro and by examining bone regeneration in a rat bone defect model. sFRP-3 treatment induced osteogenic differentiation in hMSCs as determined by alkaline phosphatase, collagen type I, osteocalcin, and Runt-related transcription factor 2 gene expression. hMSCs with or without sFRP-3 were implanted into a rat calvarial bone defect; a radiographic analysis by micro-computed tomography and histological analysis 4 and 8 weeks after implantation showed greater bone regeneration in the sFRP(+) than in the sFRP(−) group. These results suggest that modulation of Wnt signaling contributes to osteogenic differentiation in hMSCs. Specifically, sFRP-3 induces osteoblastic differentiation of cultured MSCs and bone regeneration in a calvarial bone defect, suggesting that it can be a useful agent for the treatment of bone defects. View Full-Text
Keywords: human mesenchymal stem cells (hMSCs); secreted frizzled-related protein-3 (sFRP-3); Wnt signaling; osteogenic differentiation; bone regeneration human mesenchymal stem cells (hMSCs); secreted frizzled-related protein-3 (sFRP-3); Wnt signaling; osteogenic differentiation; bone regeneration
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Katagiri, W.; Osugi, M.; Kawai, T.; Hibi, H. Secreted Frizzled-Related Protein Promotes Bone Regeneration by Human Bone Marrow-Derived Mesenchymal Stem Cells. Int. J. Mol. Sci. 2015, 16, 23250-23258.

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