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Int. J. Mol. Sci. 2014, 15(9), 16186-16195; doi:10.3390/ijms150916186

Identification of Apolipoprotein C-I as a Potential Wilms’ Tumor Marker after Excluding Inflammatory Factors

1
Department of Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China
2
Institute of Cancer, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310000, Zhejiang, China
3
Proteomic Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100000, China
*
Author to whom correspondence should be addressed.
Received: 15 July 2014 / Revised: 20 August 2014 / Accepted: 1 September 2014 / Published: 12 September 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Wilms’ tumor is one of the most common malignant tumors observed in children, and its early diagnosis is important for late-stage treatment and prognosis. We previously screened and identified protein markers for Wilms’ tumor; however, these markers lacked specificity, and some were associated with inflammation. In the current study, serum samples from children with Wilms’ tumors were compared with those of healthy controls and patients with systemic inflammatory response syndrome (SIRS). After exclusion of factors associated with inflammation, specific protein markers for Wilms’ tumors were identified. After comparing the protein peak values obtained from all three groups, a protein with a m/z of 6438 Da was specified. Purification and identification of the target protein using high-pressure liquid chromatography (HPLC) and two-dimensional liquid chromatography-linearion trap mass spectrometry(2D-LC-LTQ-MS) mass spectrometry, respectively, revealed that it was apolipoprotein C-I (APO C-I). Thus, APO C-I is a specific protein marker for Wilms’ tumor. View Full-Text
Keywords: Wilms’ tumor; systemic inflammatory response syndrome; inflammatory factor; proteomics technology; protein marker Wilms’ tumor; systemic inflammatory response syndrome; inflammatory factor; proteomics technology; protein marker
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MDPI and ACS Style

Zhang, J.; Guo, F.; Wang, L.; Zhao, W.; Zhang, D.; Yang, H.; Yu, J.; Niu, L.; Yang, F.; Zheng, S.; Wang, J. Identification of Apolipoprotein C-I as a Potential Wilms’ Tumor Marker after Excluding Inflammatory Factors. Int. J. Mol. Sci. 2014, 15, 16186-16195.

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