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Int. J. Mol. Sci. 2014, 15(9), 15061-15072; https://doi.org/10.3390/ijms150915061

Modulation of Selectin-Mediated Adhesion of Flowing Lymphoma and Bone Marrow Cells by Immobilized SDF-1

1
Department of Biomedical Engineering, Cornell University, 203 Weill Hall, Ithaca, NY 14853, USA
2
Division of Hematology/Oncology, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
*
Author to whom correspondence should be addressed.
Received: 27 June 2014 / Revised: 14 August 2014 / Accepted: 19 August 2014 / Published: 27 August 2014
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Abstract

The α-chemokine, stromal-derived factor-1 (SDF-1), has been linked to the homing of circulating tumor cells to bone. SDF-1 is expressed by bone microvascular cells and osteoblasts and normally functions to attract blood-borne hematopoietic stem and progenitor cells to marrow. It has been shown that treatment of cancer cells with soluble SDF-1 results in a more aggressive phenotype; however, the relevance of the administration of the soluble protein is unclear. As such, a flow device was functionalized with P-selectin and SDF-1 to mimic the bone marrow microvasculature and the initial steps of cell adhesion. The introduction of SDF-1 onto the adhesive surface was found to significantly enhance the adhesion of lymphoma cells, as well as low-density bone marrow cells (LDBMC), both in terms of the number of adherent cells and the strength of cell adhesion. Thus, SDF-1 has a synergistic effect with P-selectin on cancer cell adhesion and may be sufficient to promote preferential metastasis to bone. View Full-Text
Keywords: stromal-derived factor-1 (SDF-1); lymphoma; metastasis; circulating tumor cell; P-selectin; cell adhesion stromal-derived factor-1 (SDF-1); lymphoma; metastasis; circulating tumor cell; P-selectin; cell adhesion
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Hedges, E.A.; Hughes, A.D.; Liesveld, J.L.; King, M.R. Modulation of Selectin-Mediated Adhesion of Flowing Lymphoma and Bone Marrow Cells by Immobilized SDF-1. Int. J. Mol. Sci. 2014, 15, 15061-15072.

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