Next Article in Journal
New Milk Protein-Derived Peptides with Potential Antimicrobial Activity: An Approach Based on Bioinformatic Studies
Next Article in Special Issue
Farm Animal Serum Proteomics and Impact on Human Health
Previous Article in Journal
High-Resolution Imaging Reveals New Features of Nuclear Export of mRNA through the Nuclear Pore Complexes
Previous Article in Special Issue
Quantitative Proteomic Analysis of Serum Proteins from Oral Cancer Patients: Comparison of Two Analytical Methods
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(8), 14505-14530; doi:10.3390/ijms150814505

Immunoproteome of Aspergillus fumigatus Using Sera of Patients with Invasive Aspergillosis

1
Laboratory of Cellular Mycology and Proteomics, Biology Institute, University of Rio de Janeiro State (UERJ), Rio de Janeiro 20550-013, Brazil
2
Laboratory of Immunology (LIM 48), Clinics Hospital and Department of Infectious and Parasitic Diseases, Faculty of Medicine, University of São Paulo, São Paulo 05403-000, Brazil
3
Clinics of Infectious Diseases, Clinics Hospital, Faculty of Medicine, University of São Paulo, São Paulo 05403-900, Brazil
4
National Cancer Institute, Center for Bone Marrow Transplants, Rio de Janeiro 20230-130, Brazil
5
Department of Infectious and Parasitic Diseases, Faculty of Medicine, University of São Paulo, São Paulo 05403-000, Brazil
*
Author to whom correspondence should be addressed.
Received: 13 May 2014 / Revised: 24 July 2014 / Accepted: 8 August 2014 / Published: 20 August 2014
(This article belongs to the Collection Advances in Proteomic Research)
View Full-Text   |   Download PDF [1474 KB, uploaded 20 August 2014]   |  

Abstract

Invasive aspergillosis is a life-threatening lung or systemic infection caused by the opportunistic mold Aspergillus fumigatus. The disease affects mainly immunocompromised hosts, and patients with hematological malignances or who have been submitted to stem cell transplantation are at high risk. Despite the current use of Platelia Aspergillus as a diagnostic test, the early diagnosis of invasive aspergillosis remains a major challenge in improving the prognosis of the disease. In this study, we used an immunoproteomic approach to identify proteins that could be putative candidates for the early diagnosis of invasive aspergillosis. Antigenic proteins expressed in the first steps of A. fumigatus germination occurring in a human host were revealed using 2-D Western immunoblots with the serum of patients who had previously been classified as probable and proven for invasive aspergillosis. Forty antigenic proteins were identified using mass spectrometry (MS/MS). A BLAST analysis revealed that two of these proteins showed low homology with proteins of either the human host or etiological agents of other invasive fungal infections. To our knowledge, this is the first report describing specific antigenic proteins of A. fumigatus germlings that are recognized by sera of patients with confirmed invasive aspergillosis who were from two separate hospital units. View Full-Text
Keywords: immunoproteome; antigens; invasive aspergillosis; diagnosis immunoproteome; antigens; invasive aspergillosis; diagnosis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Virginio, E.D.; Kubitschek-Barreira, P.H.; Batista, M.V.; Schirmer, M.R.; Abdelhay, E.; Shikanai-Yasuda, M.A.; Lopes-Bezerra, L.M. Immunoproteome of Aspergillus fumigatus Using Sera of Patients with Invasive Aspergillosis. Int. J. Mol. Sci. 2014, 15, 14505-14530.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top