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Open AccessCommunication
Int. J. Mol. Sci. 2014, 15(8), 13932-13937; doi:10.3390/ijms150813932

Localization of MLH3 at the Centrosomes

1
Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Lines, Braunschweig 38124, Germany
2
Hannover Medical School, Division of Immunodermatology and Allergy Research, Department of Dermatology and Allergy, Hannover 30625, Germany
3
Institute of Clinical Chemistry and Laboratory Diagnostics, Heinrich-Heine-University, Medical School, Düsseldorf 40225, Germany
4
Helmholtz Centre of Infection Research, Braunschweig 38124, Germany
*
Author to whom correspondence should be addressed.
Received: 10 June 2014 / Revised: 7 July 2014 / Accepted: 4 August 2014 / Published: 11 August 2014
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases 2014)
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Abstract

Mutations in human DNA mismatch repair (MMR) genes are commonly associated with hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 protein heterodimerizes with PMS2, PMS1, and MLH3 to form MutLα, MutLβ, and MutLγ, respectively. We reported recently stable expression of GFP-linked MLH3 in human cell lines. Monitoring these cell lines during the cell cycle using live cell imaging combined with confocal microscopy, we detected accumulation of MLH3 at the centrosomes. Fluorescence recovery after photobleaching (FRAP) revealed high mobility and fast exchange rates at the centrosomes as it has been reported for other DNA repair proteins. MLH3 may have a role in combination with other repair proteins in the control of centrosome numbers. View Full-Text
Keywords: DNA mismatch repair (MMR); MLH3; centrosome DNA mismatch repair (MMR); MLH3; centrosome
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MDPI and ACS Style

Roesner, L.M.; Mielke, C.; Faehnrich, S.; Merkhoffer, Y.; Dittmar, K.E.J.; Drexler, H.G.; Dirks, W.G. Localization of MLH3 at the Centrosomes. Int. J. Mol. Sci. 2014, 15, 13932-13937.

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