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Int. J. Mol. Sci. 2014, 15(8), 13372-13387; https://doi.org/10.3390/ijms150813372

β-Defensin 2 Ameliorates Lung Injury Caused by Pseudomonas Infection and Regulates Proinflammatory and Anti-Inflammatory Cytokines in Rat

1,†
,
2
,
3
and
4,†,*
1
Department of Emergency Internal Medicine, Shanghai East Hospital, Shanghai 200120, China
2
Department of Nephrology, Shanghai East Hospital, Shanghai 200120, China
3
Department of Respiratory Medicine, Shanghai Changzheng Hospital, Shanghai 200003, China
4
Department of Respiratory Medicine, Shanghai East Hospital, Shanghai 200120, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 7 February 2014 / Revised: 13 June 2014 / Accepted: 17 June 2014 / Published: 30 July 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

An important member of the defensin family, β-defensin 2, is believed to play an important role in defense against foreign pathogens. In the present study, we constructed lentiviral vectors to express and knockdown β-defensin 2 in rat lungs. The results showed that the infection of β-defensin 2 overexpression lentivirus and β-defensin 2 shRNA effectively increased and suppressed the expression of β-defensin 2 in rat lung, respectively. The overexpression of β-defensin 2 mediated by the lentiviral vector protected lung from infection of Pseudomonas aeruginosa, but shRNA targeting β-defensin 2 aggregated the damage of lung. In addition, we also found that β-defensin 2 overexpression increased basal expression of anti-inflammatory cytokine such as IL-4, IL-10 and IL-13 and decreased levels of proinflammatory cytokines which include IL-1α, IL-1β, IL-5, IL-6, IL-8, IL-18, and TNF-α. Moreover, in the process of cytokine regulation, NF-κB pathway may be involved. Taken together, these data suggest that β-defensin 2 has protective effects against infection of Pseudomonas aeruginosa in rat and plays a role in inflammatory regulation by adjusting cytokine levels. View Full-Text
Keywords: β-defensin 2; lentiviral vector; shRNA; infection; cytokines; NF-κB β-defensin 2; lentiviral vector; shRNA; infection; cytokines; NF-κB
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Shen, Z.; Fang, L.; Zhao, L.; Lei, H. β-Defensin 2 Ameliorates Lung Injury Caused by Pseudomonas Infection and Regulates Proinflammatory and Anti-Inflammatory Cytokines in Rat. Int. J. Mol. Sci. 2014, 15, 13372-13387.

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