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Int. J. Mol. Sci. 2014, 15(7), 11996-12006; doi:10.3390/ijms150711996

Polymorphisms of Leptin-b Gene Associated with Growth Traits in Orange-Spotted Grouper (Epinephelus coioides)

1
Sanya Science & Technology Academy of Hainan National Breeding and Multiplication, Sanya 572000, China
2
Key Laboratory of Tropical Biology Resources, Ministry of Education, College of Ocean, Hainan University, Haikou 570228, China
3
Qingdao Bluegranary Science & Technology Development Center Co., Ltd., Qingdao 266000, China
4
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 25 April 2014 / Revised: 26 May 2014 / Accepted: 6 June 2014 / Published: 7 July 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

In mammals, leptin has been demonstrated to perform important roles in many physiological activities and to influence development, growth, metabolism and reproduction. However, in fish, its function is still unclear. Duplicate leptin genes, leptin-a and leptin-b, have been identified in the orange-spotted grouper. In the present study, the polymorphisms in the leptin-b gene of the orange-spotted grouper were detected, and the relation between these polymorphisms and 12 growth traits were analyzed. Six polymorphisms (including 3 single nucleotide polymorphisms (c.14G>A, c.93A>G, c.149G>A) in exon 1, 2 SNPs (c.181A>G, c.193G>A) in intron 1, and 1 SNP (c.360C>T) in exon 2) were identified and genotyped from 200 different individuals. The results revealed that the SNP c.149G>A was significantly associated with growth traits, that the heterozygous mutation genotype GA having negative effects on growth traits. However, the other five SNPs (c.14G>A, c.93A>G, c.181A>G, c.193G>A, c.360C>T) did not show significant associations with all the growth traits. Compared with our findings in leptin-a gene, the results suggested that the leptin-a hormone has more important physiological effects in fish bodies than the leptin-b type. Moreover, leptin genes were supposed to be one class of major candidate genes of regulating growth traits in the orange-spotted grouper. View Full-Text
Keywords: leptin genes; polymorphisms; genetic breeding; molecular markers; orange-spotted grouper (Epinephelus coioides); growth traits; association analysis leptin genes; polymorphisms; genetic breeding; molecular markers; orange-spotted grouper (Epinephelus coioides); growth traits; association analysis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Huang, H.; Wei, Y.; Meng, Z.; Zhang, Y.; Liu, X.; Guo, L.; Luo, J.; Chen, G.; Lin, H. Polymorphisms of Leptin-b Gene Associated with Growth Traits in Orange-Spotted Grouper (Epinephelus coioides). Int. J. Mol. Sci. 2014, 15, 11996-12006.

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