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Int. J. Mol. Sci. 2014, 15(7), 11713-11729; doi:10.3390/ijms150711713
Article

The Involvement of miR-23a/APAF1 Regulation Axis in Colorectal Cancer

1,* , 2
, 1
 and 1
1 Department of Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia 2 Department of Biochemistry, Faculty of Medicine, MAHSA University, Kuala Lumpur 59100, Malaysia
* Author to whom correspondence should be addressed.
Received: 13 May 2014 / Revised: 2 June 2014 / Accepted: 4 June 2014 / Published: 2 July 2014
(This article belongs to the Special Issue Regulation by non-coding RNAs 2013)
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Abstract

Recent advances in microRNAome have made microRNAs (miRNAs) a compelling novel class of biomarker in cancer biology. In the present study, the role of miR-23a in the carcinogenesis of colorectal cancer (CRC) was investigated. Cell viability, apoptosis, and caspase 3/7 activation analyses were conducted to determine the potentiality of apoptosis resistance function of miR-23a in CRC. Luciferase assay was performed to verify a putative target site of miR-23a in the 3'-UTR of apoptosis protease activating factor 1 (APAF1) mRNA. The expression levels of miR-23a and APAF1 in CRC cell lines (SW480 and SW620) and clinical samples were assessed using reverse transcription-quantitative real-time PCR (RT-qPCR) and Western blot. We found that the inhibition of miR-23a in SW480 and SW620 cell lines resulted in significant reduction of cell viability and promotion of cell apoptosis. Moreover, miR-23a up-regulation was coupled with APAF1 down-regulation in CRC tissue samples. Taken together, miR-23a was identified to regulate apoptosis in CRC. Our study highlights the potential application of miR-23a/APAF1 regulation axis in miRNA-based therapy and prognostication.
Keywords: miR-23a; APAF1; colorectal cancer; apoptosis; SW480; SW620 miR-23a; APAF1; colorectal cancer; apoptosis; SW480; SW620
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yong, F.L.; Wang, C.W.; Roslani, A.C.; Law, C.W. The Involvement of miR-23a/APAF1 Regulation Axis in Colorectal Cancer. Int. J. Mol. Sci. 2014, 15, 11713-11729.

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