New Coumarins and Anti-Inflammatory Constituents from the Fruits of Cnidium monnieri
Abstract
:1. Introduction
2. Results
Compounds | Superoxide Anion | Elastase |
---|---|---|
IC50 [µg/mL] b or (Inh %) c | ||
3'-O-Methylmurraol (1) | 6.24 ± 0.50 g | (35.10 ± 5.71) f |
1'-O-Methylphlojodicarpin (2) | (15.04 ± 1.26) g | (6.02 ± 1.56) e |
1'-O-Methylvaginol (3) | (11.99 ± 2.14) f | (6.42 ± 2.07) e |
Murraol (4) | 2.83 ± 0.46 f | (18.16 ± 5.62) e |
Peroxymurraol (5) | 2.87 ± 0.11 g | (5.78 ± 2.98) |
Osthol (6) | 0.005 ± 0.0002 f | (31.96 ± 5.72) f |
Osthenol (7) | 0.09 ± 0.01 g | 3.28 ± 0.90 g |
Auraptenol (8) | 0.77 ± 0.11 e | (22.46 ± 2.65) f |
Peroxyauraptenol (9) | 0.41 ± 0.06 e | 7.83 ± 1.17 e |
Meranzin hydrate (10) | 7.31 ±1.62 e | 4.21 ± 1.41 e |
Demethylauraptenol (11) | 0.54 ± 0.05 g | 4.36 ± 1.67 f |
Xanthotoxin (12) | 0.32 ± 0.13 e | (14.81 ± 6.03) |
Xanthotoxol (13) | (24.54 ± 2.47) g | (42.94 ± 4.29) g |
Imperatorin (14) | 0.07 ± 0.02 g | (22.08 ± 2.93) f |
Bergapten (15) | 0.36 ± 0.09 e | 4.62 ± 1.36 e |
Isopimpinellin (16) | 2.75 ± 0.26 | (8.97 ± 2.28) e |
Cnidimol A (17) | 3.65 ± 0.41 g | 3.20 ± 0.16 g |
Diphenyleneiodonium d | 0.55 ± 0.20 g | - |
Phenylmethylsulfonyl fluoride d | - | 34.4 ± 5.5 g |
3. Discussion
4. Experimental Section
4.1. Ethics Statement
4.2. General Experimental Procedures
4.3. Plant Material
4.4. Extraction and Isolation
4.4.1. 3'-O-Methylmurraol (1)
4.4.2. rel-(1'S,2'S)-1'-O-Methylphlojodicarpin (2)
4.4.3. (1'S,2'S)-1'-O-Methylvaginol (3)
4.4.4. Supplementary Files
4.5. Biological Assay
4.5.1. Preparation of Human Neutrophils
4.5.2. Measurement of Superoxide Anion Generation
4.5.3. Measurement of Elastase Release
4.5.4. Statistical Analysis
5. Conclusions
Supplementary Files
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lee, T.-H.; Chen, Y.-C.; Hwang, T.-L.; Shu, C.-W.; Sung, P.-J.; Lim, Y.-P.; Kuo, W.-L.; Chen, J.-J. New Coumarins and Anti-Inflammatory Constituents from the Fruits of Cnidium monnieri. Int. J. Mol. Sci. 2014, 15, 9566-9578. https://doi.org/10.3390/ijms15069566
Lee T-H, Chen Y-C, Hwang T-L, Shu C-W, Sung P-J, Lim Y-P, Kuo W-L, Chen J-J. New Coumarins and Anti-Inflammatory Constituents from the Fruits of Cnidium monnieri. International Journal of Molecular Sciences. 2014; 15(6):9566-9578. https://doi.org/10.3390/ijms15069566
Chicago/Turabian StyleLee, Tzong-Huei, Yuan-Chih Chen, Tsong-Long Hwang, Chih-Wen Shu, Ping-Jyun Sung, Yun-Ping Lim, Wen-Lung Kuo, and Jih-Jung Chen. 2014. "New Coumarins and Anti-Inflammatory Constituents from the Fruits of Cnidium monnieri" International Journal of Molecular Sciences 15, no. 6: 9566-9578. https://doi.org/10.3390/ijms15069566