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Int. J. Mol. Sci. 2014, 15(6), 9481-9496; doi:10.3390/ijms15069481
Article

A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma

1,2
, 3,* , 1,* , 4,* , 3
 and 5
1 Department of Lymphoma, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China 2 Department of Blood Transfusion, Jinan Military General Hospital, Jinan 250031, China 3 Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin Shengfa NabioTech Co., Ltd., Tianjin 300457, China 4 Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China 5 Division of Science Popularization, China Science and Technology Exchange Center, Beijing 100045, China
* Authors to whom correspondence should be addressed.
Received: 21 April 2014 / Revised: 13 May 2014 / Accepted: 14 May 2014 / Published: 28 May 2014
(This article belongs to the Section Molecular Pathology)
View Full-Text   |   Download PDF [647 KB, 19 June 2014; original version 19 June 2014]   |   Browse Figures

Abstract

Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
Keywords: immune VHH phage display library; B cell-activating factor; sdAb immune VHH phage display library; B cell-activating factor; sdAb
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wu, W.; Li, S.; Zhang, W.; Sun, J.; Ren, G.; Dong, Q. A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma. Int. J. Mol. Sci. 2014, 15, 9481-9496.

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