Abstract: Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
Keywords: immune VHH phage display library; B cell-activating factor; sdAb
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Wu, W.; Li, S.; Zhang, W.; Sun, J.; Ren, G.; Dong, Q. A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma. Int. J. Mol. Sci. 2014, 15, 9481-9496.
Wu W, Li S, Zhang W, Sun J, Ren G, Dong Q. A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma. International Journal of Molecular Sciences. 2014; 15(6):9481-9496.
Wu, Wen; Li, Shenghua; Zhang, Weijing; Sun, Jian; Ren, Guangda; Dong, Quanchao. 2014. "A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma." Int. J. Mol. Sci. 15, no. 6: 9481-9496.