Abstract: Objective: To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Methods: Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA). Results: We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro. Conclusion: The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Wu, W.; Li, S.; Zhang, W.; Sun, J.; Ren, G.; Dong, Q. A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma. Int. J. Mol. Sci. 2014, 15, 9481-9496.
Wu W, Li S, Zhang W, Sun J, Ren G, Dong Q. A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma. International Journal of Molecular Sciences. 2014; 15(6):9481-9496.
Wu, Wen; Li, Shenghua; Zhang, Weijing; Sun, Jian; Ren, Guangda; Dong, Quanchao. 2014. "A Novel VHH Antibody Targeting the B Cell-Activating Factor for B-Cell Lymphoma." Int. J. Mol. Sci. 15, no. 6: 9481-9496.